8,883 results

Showing: 6641 - 6680 of 8883 results

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pcDNA5/FRT/TO GFP HSPA1A

Plasmid

#19483

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1A (HSPA1A Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLuc-CDS

Plasmid

#42100

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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mPlum-C1

Plasmid

#54839

Purpose

Localization: C1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pJ3M-Mst2

Plasmid

#12205

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst2 (STK3 Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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T4 Lysozyme WT*

Plasmid

#18111

Depositor

Brian Matthews

Insert

T4 Lysozyme (e Bacteriophage T4)

Expression

Bacterial

Mutation

No cysteines: C54T, C97A

Available Since

Aug. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (P#468)

Plasmid

#14607

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (CCNT1 Human)

Tags

GST

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pMS2-YFP

Plasmid

#27122

Depositor

Robert Singer

Article

Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

Insert

MS2

Tags

EYFP, HA, and SV40 NLS

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe neo IRES-EGFP

Plasmid

#14431

Depositor

L. Miguel Martins

Insert

IRES-EGFP

Use

Retroviral

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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RSV-Sp1

Plasmid

#12098

Depositor

Robert Tjian

Insert

Sp1 (SP1 Human)

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H-Ras_V12

Plasmid

#39504

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-beta-catenin-S33Y

Plasmid

#13371

Purpose

Retroviral expression of mouse beta-catenin S33Y mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 33 to Tyrosine and Leucine 388 to Proline

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAM1

Plasmid

#51401

Purpose

Expresses truncated version of NLL-MetRS from endogenous MetG promoter

Depositor

David Tirrell

Article

Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Insert

MetRS (metG E coli)

Mutation

L13N, Y260L, H301L; Please note that a truncated …

Promoter

MetG

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CUL4A

Plasmid

#19907

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Cullin 4A (CUL4A Human)

Tags

2xHA

Expression

Mammalian

Mutation

K644R

Promoter

CMV

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro

Plasmid

#141380

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-YTB

Plasmid

#26721

Depositor

Edward Callaway

Article

Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

Insert

venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))

Expression

Mammalian

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pGL2B-HIF1A prom

Plasmid

#40172

Depositor

Alex Minella

Article

Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

Insert

HIF1A promoter fragment (HIF1A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2::VP16Uw

Plasmid

#26228

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::VP16 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINM

Plasmid

#26044

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
GFP Snail WT

Plasmid

#16225

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Pomc-pGL3

Plasmid

#17553

Purpose

Luciferase expression under control of Pomc promoter (–646 to +65).

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Pomc promoter (Pomc Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

Pomc

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-22b DT 51E/148K

Plasmid

#11081

Depositor

John Collier

Insert

DT

Tags

His

Expression

Bacterial

Mutation

51E/148K, catalytically inactive

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pHYRS52

Plasmid

#31122

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

Ulp1 (ULP1 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

Ulp1(403-621)

Available Since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM3Dq

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pYES2/103Q

Plasmid

#1385

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (103Q) (HTT Human)

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/HisMaxB-YAP1-S127A

Plasmid

#18988

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S127A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 127 changed to Alanine, mutated protein t…

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pWZL-Blast-Flag-HA-hTERT

Plasmid

#22396

Depositor

William Hahn

Article

Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

Insert

TERT (TERT Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
413 pSG5L HA RB

Plasmid

#10720

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (RB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9m4-VP64

Plasmid

#47319

Purpose

Cas9m4 Activator

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4-VP64

Use

CRISPR

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,883 results