Skip to main content
Addgene
Showing: 6681 - 6720 of 10228 results

  1. pBSFI-myr-akt-T308A

    Plasmid
    #49189
    Purpose
    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector
    Depositor
    Peter Vogt
    Article
    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(
    Insert
    Akt1 (Akt1 Mouse)
    Use
    Adaptor plasmid
    Tags
    HA tag and myristolation signal
    Expression
    Mutation
    T308A
    Promoter
    T7
    Available since
    Dec. 13, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pET-30a(+)-Syk-tSH2-PM

    Plasmid
    #111271
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y130E mutation to mimic phosphorylation
    Promoter
    T7 promoter
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111271-plasmid-map-sequence-id-215610

    Enlarge

  10. pStr-KDEL_EPV-SBP-DsRed2-ER-5

    Plasmid
    #170676
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    EPV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    IRES and T7
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170676-plasmid-map-sequence-id-335802

    Enlarge

  15. pBS-Nvec-codonopti-Cas9-2xNLS

    Plasmid
    #141108
    Purpose
    Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis
    Depositor
    Matthew Gibson
    Article
    Hill et al Methods Mol Biol. 2022;2450:437-465. doi
    Insert
    Nematostella codon optimized Cas9
    Use
    Unspecified
    Tags
    Expression
    Mutation
    All amino acids coded for by codons optimized for…
    Promoter
    T7
    Available since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    141108-plasmid-map-sequence-id-314929

    Enlarge

  22. pET28a Gx-NL3

    Plasmid
    #169108
    Purpose
    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169108-plasmid-map-sequence-id-335659

    Enlarge

Showing: 6681 - 6720 of 10228 results