178,651 results
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Plasmid#106073Purposeexpress the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based ACh sensor (GACh) 2.0
ExpressionMammalianPromoterCMVAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
Plasmid#104567PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
UseCRISPRTagsNLS-3xHA-VPRExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ezh2[FL]-dCas9
Plasmid#100086PurposeFull-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert14056 (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[FL]-dCas9-NLSExpressionMammalianPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-GAL4-ERT2-VP16
Plasmid#82588PurposeMiddle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAL4-ERT2-VP16
PromoterNoneAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB554
Plasmid#74884PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK alpha 1 (PRKAA1 Human)
ExpressionMammalianPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28b-BE1
Plasmid#73018PurposeExpresses BE1 with N-terminal His6 tag in E. ColiDepositorInsertBE1
TagsHis6 tagExpressionBacterialPromoterT7Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK294 (mAID-mCherry2-Bsr)
Plasmid#72832PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mCherry2-Bsr
UseCRISPRTagsmAID-mCherry2ExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ornithine transcarbamylase (OTC)
Plasmid#71877PurposeFor mitochondrial unfolded protein response researchDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOTC (Otc Rat)
TagsNoneExpressionMammalianPromoterCAGAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-PGK
Plasmid#72268PurposeExpress gene of interest under PGK (phosphoglycerate kinase I) promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterPGKAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
ExpressionMammalianAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_TNF-SBP-EGFP
Plasmid#65278Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNF (TNF Human)
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-PBRM1
Plasmid#65387PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPBRM1 (PBRM1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRD3
Plasmid#65377PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRD3 (BRD3 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVExpressionMammalianPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsmCerulean3ExpressionMammalianPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Beta-Catenin-20
Plasmid#55001PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta-Catenin (Ctnnb1 Mouse)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1
Plasmid#58437PurposeExpresses LexA-ER-B112 in yeast cellsDepositorInsertLexA-ER-B112
ExpressionYeastPromoterAct1 promoter (-520;1)Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS-H2B-mRFP1
Plasmid#53745PurposeContains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone H2B (H2BC21 Human)
TagsmRFPExpressionMammalianPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKeima-Red-C1
Plasmid#54546PurposeLocalization: C1 Cloning Vector, Excitation: 440, Emission: 620DepositorTypeEmpty backboneTagsmKeima-RedExpressionMammalianAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-EF-FH-TAZ S89A-ires-blast
Plasmid#52084PurposeLentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tagsDepositorPublicationInsertTAZ S89A (WWTR1 Human)
UseLentiviralTagsFLAG and HisExpressionMammalianMutationS89APromoterEF1aAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7-TPase
Plasmid#51818PurposeFor in vitro transcription of Tol2 transposase using T7 PromoterDepositorInsertTol2 Transposase
UseUnspecifiedPromoterT7Available sinceApril 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SrtAdelta59
Plasmid#51138Purposeexpression plasmid for N-terminal his tagged S.aureus SrtA delta 59DepositorInsertS.aureus SrtA delta 59
Tags6x HisExpressionBacterialPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-MAP3K5 (WT)
Plasmid#47104PurposeExpresses wild type human MAP3K5 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAP3K5 (MAP3K5 Human)
TagsFLAGExpressionMammalianMutationNAPromoterCMVAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by