8,732 results

1. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

   Plasmid

   #166055

   Purpose

   Expresses the full length TurboID on the surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDx_mScarlet-I3

   Plasmid

   #189757

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNAPf-ADRβ2 Control Plasmid

   Plasmid

   #101123

   Purpose

   Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   Beta-2-Adrenergic Receptor (ADRB2 Human)
   

   Use

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-hM3D(Gq)-mCherry

   Plasmid

   #50474

   Purpose

   Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. psfaRb-Tn5

   Plasmid

   #183638

   Purpose

   Expresses anti-rabbit nanobody fused with Tn5

   Depositor

   Goncalo Castelo-Branco

   Article

   Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

   Insert

   anti rabbit secondary nanobody fused to Tn5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-hALIX

   Plasmid

   #21504

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   ALIX (PDCD6IP Human)
   

   Use

   Tags

   Cherry

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV rtTA3 Blast (w756-1)

   Plasmid

   #26429

   Purpose

   3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor A3 mutant
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   8X Gli-binding site

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC407 - pAAV EF1a eGFP

    Plasmid

    #60058

    Purpose

    An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    enhanced Green Fluorescent Protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

    Plasmid

    #167582

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRE recombinase

    Plasmid

    #62730

    Purpose

    Expresses Cre recombinase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-CD14

    Plasmid

    #13645

    Depositor

    Doug Golenbock

    Insert

    CD14 (CD14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEN2-SG4

    Plasmid

    #196071

    Purpose

    Express wild type SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 RLUC POLIRES FLUC

    Plasmid

    #45642

    Purpose

    Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

    Depositor

    Nahum Sonenberg

    Article

    Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

    Insert

    RLUC - IRES - FLUC
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBC-DT1T2

    Plasmid

    #50590

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, U626t plus, U629p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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