8,789 results

1. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINDUCER21 (ORF-EG)

   Plasmid

   #46948

   Purpose

   Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

   Depositor

   Thomas Westbrook, Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE7

   Plasmid

   #214812

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV Puro

   Plasmid

   #68469

   Purpose

   Retroviral plasmid with multiple cloning site and puromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Puromycin Resistance
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Kras-Src FRET biosensor

   Plasmid

   #78302

   Purpose

   FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

   Insert

   Src biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Rab7A T22N

   Plasmid

   #28048

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   T22N

   Promoter

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-TREX1

   Plasmid

   #27219

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available Since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. MP6

    Plasmid

    #69669

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. APEX2-NLS

    Plasmid

    #124617

    Purpose

    proximity biotinylation

    Depositor

    Alice Ting

    Article

    Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

    Insert

    APEX2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6-H2B-mCherry

    Plasmid

    #89766

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    Histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTNT-B18R

    Plasmid

    #58978

    Purpose

    expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Sp6

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSGKP-km

    Plasmid

    #117233

    Purpose

    A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid

    #177316

    Purpose

    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

    Depositor

    Hirokazu Hirai

    Article

    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

    Insert

    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Use

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pE-FLP

    Plasmid

    #45978

    Purpose

    Expresses yeast Flp driven by the constitutive promoter pE from phage P2

    Depositor

    Drew Endy, Keith Shearwin

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Insert

    flp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    flp driven by the constitutive promoter pE from p…

    Promoter

    pE (from phage P2)

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRX1 flag

    Plasmid

    #21283

    Depositor

    Wang Min

    Article

    Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

    Insert

    TRX1 (TXN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-EF1a-IRES-Blast

    Plasmid

    #85133

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti CMV Hygro DEST (w117-1)

    Plasmid

    #17454

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB-TO-oNGN2

    Plasmid

    #198397

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

    Promoter

    CAG, EF1a, and TRE3G

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1-blast

    Plasmid

    #26655

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MCS-BioID2-HA

    Plasmid

    #74224

    Purpose

    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    HA and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mito-BFP

    Plasmid

    #49151

    Purpose

    visualization of mitochondria

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    mitochondrial targeting signal (COX4 Budding Yeast)
    

    Use

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EGFP-BRCA1

    Plasmid

    #115491

    Purpose

    EGFP-BRCA1 expression vector. Parton clone BXY

    Depositor

    Rob Parton

    Article

    McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1009R (See depositor comments)

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Use

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Promoter

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mCherry-Mito-7

    Plasmid

    #55102

    Purpose

    Localization: Mitochondria, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTALdNC-AAVS1_T1

    Plasmid

    #80495

    Purpose

    AAVS1 TALEN expression vector (Left)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Left)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTALdNC-AAVS1_T2

    Plasmid

    #80496

    Purpose

    AAVS1 TALEN expression vector (Right)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 TALEN (Right)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV_hEF1a_rtTA3

    Plasmid

    #61472

    Purpose

    3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Insert

    rtTA3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hEF1a

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge