We narrowed to 15,050 results for: NOL
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Plasmid#192397PurposeTo test if a single plasmid stably transformed into Arabidopsis can switched from off to on when its AND gate unit is activated by both cell type (cortex) and chemical induction (by DEX).DepositorInsert35S::FRT-OCS-FRT::B3RT-OCS-B3RT::Turbo GFP
UseSynthetic BiologyTagsN7ExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)
Plasmid#191165PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTRDepositorInsertmCherry:s70587-3xMS2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc0
Plasmid#186771PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hNluc
Plasmid#186772PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::hNluc
Plasmid#186774PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc1
Plasmid#186754PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL10::sNluc1
Plasmid#186756PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc1
Plasmid#186757PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc0
Plasmid#186758PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc2
Plasmid#186759PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc3
Plasmid#186760PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc4
Plasmid#186761PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::sNluc5
Plasmid#186762PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc2_STOP
Plasmid#186767PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.DepositorInsertNanoluciferase
UseTagsExpressionMutationPremature stop codon at residue 16PromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo-W35A
Plasmid#179397PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)DepositorInsertCBX7_2xChromo(W35A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_3EGFP
Plasmid#185967PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPr-Egfp cassette in forward direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo-W42A
Plasmid#179394PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)DepositorInsertCBX1_2xChromo(W42A)
UseTagsAviTag and EGFPExpressionMammalianMutationChromodomain W42APromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv7_MBD1_MBD_noEGFP
Plasmid#179406PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv7_CBX1_2xChromo_noEGFP
Plasmid#179395PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFPDepositorInsertCBX1_2xChromo_noEGFP
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD-R22A
Plasmid#179405PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)DepositorInsertMBD1_MBD(R22A)
UseTagsAviTag and EGFPExpressionMammalianMutationMBD R22APromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1010A
Plasmid#176078PurposepBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010B
Plasmid#176079PurposepBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010C
Plasmid#176080PurposepBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010D
Plasmid#176081PurposepBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC51
Plasmid#104825PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoterDepositorInsertGlyma.12g075700, Glyma.11g145900
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265C
Plasmid#164715PurposeGateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editingDepositorInserthAID-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible haid-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E1
Plasmid#164718PurposeGateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editingDepositorInsertA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible a3a-cbe_v01 entry cloneTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E3
Plasmid#164720PurposeGateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editingDepositorInserteA3A-zCas9(D10A)-UGI
UseCRISPR; Gateway compatible ea3a-cbe_v01 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:AsCas12a_DR (GB1442)
Plasmid#160574PurposeAsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:AsCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 LbCas12a (GB1439)
Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AsCas12a (GB1438)
Plasmid#160572PurposeHuman codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).DepositorInsertAsCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc227
Plasmid#161948PurposeContains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on TetRDepositorInsertpSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR(1-20)-BsaI-BsaI-TetR(188-207)-Ter
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc367
Plasmid#161947PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20DepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc228
Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-ZC3H12D_PromoterWT
Plasmid#153065PurposeZC3H12D promoter region cloned upstream of luciferase reporter geneDepositorInsertZC3H12D promoter region (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterNoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pK_lacO-cP-AOX1-AtX+NpgA
Plasmid#126727Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG
Plasmid#127309PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127317PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2
Plasmid#126734Purposeyeast plasmid overexpressing ADH2 from Saccharomyces cerevisiaeDepositorInsertadh2
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_-P-GAP-NpgA+AtX
Plasmid#126725Purposeyeast plasmid overexpressing AtX and NpgADepositorInsertatX,npgA
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6
Plasmid#126737Purposeyeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces cerevisiaeDepositorInsertadh2,ald6
UseTags6xHisExpressionYeastMutationPromoterGAPAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Mit1AD
Plasmid#126721PurposeLacI-Mit1AD on pPICZ B expression vectorDepositorInserthybrid lacI-Mit1 activation domain coding sequence
UseTagsExpressionYeastMutationPromoterICL1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-VP16
Plasmid#126723PurposeLacI-VP16 on pPICZ B expression vectorDepositorInserthybrid lacI-VP16 activation domain coding sequence
UseTagsExpressionYeastMutationPromoterICL1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-ICL1-LacI-Gal4AD
Plasmid#126724PurposeLacI-Gal4AD on pPICZ B expression vectorDepositorInserthybrid lacI-Gal4 activation domain coding sequence
UseTagsExpressionYeastMutationPromoterICL1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-sLovA
Plasmid#126741Purposeyeast plasmid overexpressing sLovADepositorInsertslovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii
UseTags6xHisExpressionYeastMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG
Plasmid#127325PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST
Plasmid#127320PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)DepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST
Plasmid#127313PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only