We narrowed to 21,605 results for: ESC
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Plasmid#197153PurposeExpresses the protein of iRFP682-P2A-GFP in mammalian cellsDepositorInsertiRFP682-P2A-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry2-ER
Plasmid#164166PurposeExpresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoterDepositorInsertmCherry2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-P2A-ZmWus2-9
Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_TNF-SBP-EGFP
Plasmid#65280Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJT195
Plasmid#202294PurposeType IIS cloning plasmid for LPattB7 integrations w/ OriTDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisTagsExpressionMutationPromoter8xGliBS-delta-crystallin minimal promoterAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-GPI
Plasmid#65295Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn-FLEX.iGABASnFR.F102G
Plasmid#112164PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR.F102G
UseAAVTagsExpressionMutationF102GPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG TORC1 (1-634)
Plasmid#25718DepositorInsertTORC1 (1-634) (CRTC1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS TIR-2 sfGFP
Plasmid#154468PurposepET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
Plasmid#126552PurposeReplication incompetent HIV with H13L Tat and d2eGFP/CD8a reporterDepositorInsertHIV-1 vector pNL4-3
UseLentiviralTagsCD8a and GFPExpressionMutationDelta gag/polPromoterHIV LTRAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express2
Plasmid#103999PurposePlasmid encoding DsRed-Express2DepositorInsertDsRed-Express2
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet3_N1
Plasmid#189778PurposeConstruct for testing oligimerization of mScarlet3 in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.661
Plasmid#108532PurposeTtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.DepositorInsertTtgRAM + PTtg-YFP
UseTagsExpressionBacterialMutationTtgR: R130H A144T A148VPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-200
Plasmid#79602PurposeFUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sitesDepositorInsertd2GFP + miR-200 family targets
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneUseTags6xHis and mKate1.31ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMUT2-pTcpP-mCherry
Plasmid#192861PurposepMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917DepositorInsertTcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
UseTagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDAS12125_PEAR-GFP
Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.cytGAP
Plasmid#78734PurposeExpression of Ca2+ sensor GAP in the cytosolDepositorInsertcytGAP
UseTagsluciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-T2A-moxMaple3
Plasmid#120875PurposeMammalian expression of mCherry and moxMaple3DepositorInsertsmCherry
moxMaple3
UseTagsExpressionMammalianMutationfrom mMaple3, moxMaple3 was engineered by replaci…PromoterAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Calreticulin-N-10
Plasmid#57353PurposeLocalization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertCalreticulin (CALR Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
UseTagsExpressionBacterialMutationPromotercda SOS promoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationdeleted n' 15 amino acidsPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Lyn-PlcR
Plasmid#181830PurposeGenetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.DepositorInsertLyn-PlcR
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA7-mRuby3
Plasmid#184893PurposeStable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cellsDepositorInsertANXA7 (ANXA7 Human)
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.884
Plasmid#108536PurposeAcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.DepositorInsertAcuRAM + PAcu-YFP
UseTagsExpressionBacterialMutationAcuR: E80K R154H L202MPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
UseTagsExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046899
Plasmid#145608PurposeBacterial Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-Electra2
Plasmid#179477PurposeER Visualization (OSER assay) Expresses Electra2 in fusion with N-terminal part of p450DepositorInsertCytERM-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H TC editing reporter
Plasmid#198882PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93HPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag
Plasmid#209658PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGECInano
Plasmid#186188PurposeAAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicatorDepositorInsertiGECInano
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230058PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS
Plasmid#230059PurposeExpresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.DepositorInsertAnti-GluA2 single chain variable fragment (scFv)
UseTags6X HIS, HRV 3C PreScission protease, HaloTag, and…ExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-mCherry
Plasmid#65256Purposesynchronize trafficking of ManII from the ERDepositorInsertIi-Streptavidin and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-epNTR
Plasmid#62213PurposeepNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2DepositorInsertepNTR
UseMrna synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-Lysosomes-20
Plasmid#55902PurposeLocalization: Lysosome Membrane, Excitation: 559, Emission: 600DepositorInsertLAMP1
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorInsertCrowdedness-sensitive linker domain
UseTagsmClover2 and mRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only