We narrowed to 10,975 results for: NSI;
-
Plasmid#45709DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationT787KPromoterAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBp-FGFR2b-E471Q
Plasmid#45703DepositorInsertFGFR2 IIIb (FGFR2 Human)
UseRetroviralTagsExpressionMammalianMutationE471QPromoterAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRCA816 - pBA904 Puro-T2A-GFP ZNF296 g6 CRISPRa guide (pRCA360 backbone) 694
Plasmid#238179PurposeLentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertZNF296 sgRNA CRISPRa (ZNF296 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA815 - pBA904 Puro-T2A-GFP ZNF296 g5 CRISPRa guide (pRCA360 backbone) 695
Plasmid#238178PurposeLentiviral CRISPR guide vector expressing a ZNF296 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertZNF296 sgRNA CRISPRa (ZNF296 Synthetic, Human)
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-Digda
Plasmid#236102PurposeLysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.DepositorInsertLyso-Digda (LAMP1 Synthetic, Human)
UseTagsFull-length LAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p300dAIL KAT LIC 1M
Plasmid#233588PurposeBacterial expression of p300 KAT enzyme with truncated autoinhibitory loopDepositorInsertp300dAIL KAT (EP300 Human)
UseTags6xHis MBPExpressionBacterialMutationdeleted amino acids 1523-1554PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-CHST8
Plasmid#232650Purposeexpresses CHST8 wild-type in mammalian cellsDepositorInsertCHST8 (CHST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-CHST8 (3A)
Plasmid#232651Purposeexpresses CHST8 REP-to-AAA mutant in mammalian cellsDepositorInsertCHST8 (3A) (CHST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR258A, E259A, P260APromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-CD274-Nluc
Plasmid#232658PurposeNluc-tagged PD-L1 expresion in mammalian cells; lectin-captured luciferase reporter assayDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
Scon_PheRS_TP_pK7fWG2_IVD_FP611
Plasmid#228372PurposeExpresses N-term transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from Silene conica PheRS (Warren et al., 2023) with demonstrated plastid targeting.
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228374PurposeExpresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_CytC_87.18_SHI04112_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228375PurposeExpresses N-term transit peptide from Sapria himalayana Cytochrome C with demonstrated mito targeting; proof-of-concept for Plasmid 228371DepositorInsertN-terminal transit peptide from S. himalayana Cytochrome C with demonstrated mito targeting.
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_GluRS_87.31.3_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228376PurposeExpresses N-term transit peptide from Sapria himalayana Glutamate aminoacyl-tRNA synthetase evm.model.fragScaff_scaffold_87.31.3; determination of S. himalayana GluRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana GluRS evm.model.fragScaff_scaffold_87.31.3
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_MetRS_440.109_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228378PurposeExpresses N-term transit peptide from Sapria himalayana Methionine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 440.10; determination of S. himalayana MetRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana MetRS evm.model.fragScaff scaffold 440.10
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_ThrRS_3895.3_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228380PurposeExpresses N-term transit peptide from Sapria himalayana Threonine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3895.3; determination of S. himalayana ThrRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana ThrRS evm.model.fragScaff scaffold 3895.3
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611
Plasmid#228381PurposeExpresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localizationDepositorInsertN-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG
Plasmid#222854PurposeMammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 FcDepositorInsertAT118-L
UseTagsIgG1_FcExpressionMammalianMutationL234A, L235A, P329GPromoterAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCA528 huPQBP1
Plasmid#217449PurposeExpresses full-length human PQBP1 from a His-SUMO bacterial expression vectorDepositorInsertPQBP1 (PQBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA E45A
Plasmid#217438PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA E45APromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA M66I
Plasmid#217440PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with M66I mutation in CA).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA M66IPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
SG3ΔENV CA Q67H/N74D
Plasmid#217442PurposeNon-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).DepositorInsertgag/pol
UseLentiviralTagsExpressionMutationCA Q67H,N74DPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyOAHIDS
Plasmid#226246PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApySDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyOAHID
Plasmid#226245PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHIDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
UseTagsHexa-HistidineExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-MYC-As-NF-kB3
Plasmid#180205PurposeExpresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cellsDepositorInsertAs-NF-kB3
UseTagsMYC-tagged fusion proteinExpressionMammalianMutationaa 2-412PromoterCMVAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD86-His10
Plasmid#223570PurposeProtein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorInsertCD86 (CD86 Human)
UseTagsHis6 and Twin-Strep, SNAPExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA K230A
Plasmid#210814PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAExpressionMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-As-NF-kB2
Plasmid#180201PurposeExpresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cellsDepositorInsertAs-NF-kB2
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-502PromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL43C
Plasmid#218278PurposeIntroduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL4A
Plasmid#218277PurposeIntroduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL3C
Plasmid#218275PurposeIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP703-eDHFR(69K6)-cRaf
Plasmid#184717PurposeiRafDepositorInsertmiRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA K230A
Plasmid#210808PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAExpressionMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE ∆238-245
Plasmid#139318PurposeGST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
UseTagsGSTExpressionBacterialMutationAO7 cloned into the first multiple cloning site o…PromoterT7 promoter-1Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-I248V
Plasmid#201554PurposeExpresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationchanged Isoleucine 248 to Valine (numbering based…PromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
hARL15_32-197
Plasmid#209799PurposeBacterial expression of human ARL15 GTPase domainDepositorInsertARL15 (ARL15 Human)
UseTags6xHisExpressionBacterialMutationaa 32-197 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)
Plasmid#209776PurposeCRISPRi for STK4DepositorInsertNluc, sgSTK4i, mRuby2 (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)
Plasmid#209773PurposeCRISPRi for STK4DepositorInsertdCas9-KRAB, sgSTK4i (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)
Plasmid#209774PurposeCRISPRi for STK4DepositorInsertdCas9-KRAB, sgSTK4i (STK4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA
Plasmid#174895Purposeamber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in sfGFPPromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only