Skip to main content
Addgene
Showing: 8751 - 8800 of 157954 results

  8. TFORF2860

    Plasmid
    #143643
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ID1 (ID1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143643-plasmid-map-sequence-id-392313

    Enlarge

  10. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

    Enlarge

  13. pKL06

    Plasmid
    #104430
    Purpose
    super Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector
    Depositor
    Joel Kralj
    Article
    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10
    Insert
    Super Ecliptic pHluorin-mRuby2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    104430-plasmid-map-sequence-id-247766

    Enlarge

  19. B-GRAM-W

    Plasmid
    #211707
    Purpose
    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
    Depositor
    Yasunori Saheki
    Article
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211707-plasmid-map-sequence-id-419219

    Enlarge

  29. Dendra2-Lifeact-7

    Plasmid
    #54694
    Purpose
    Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Dendra2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54694-plasmid-map-sequence-id-138985

    Enlarge

  31. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

    Enlarge

  34. hSYN-mito-dsRED2

    Plasmid
    #173069
    Purpose
    mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Lentiviral
    Tags
    dsRED
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    173069-plasmid-map-sequence-id-345051

    Enlarge

  41. pAAV-nEF-Con/Fon-ChRmine-oScarlet

    Plasmid
    #137159
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Fon-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137159-plasmid-map-sequence-id-272775

    Enlarge

  42. p663-UBC-miniTurbo-V5-PKN3_IDG-K

    Plasmid
    #170601
    Purpose
    Gateway destination clone of PKN3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-PKN3 (PKN3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170601-plasmid-map-sequence-id-335768

    Enlarge

  43. pcDNA3-ps-CFP

    Plasmid
    #13034
    Purpose
    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence
    Depositor
    Doug Golenbock
    Insert
    photo-switchable cyan fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    13034-plasmid-map-sequence-id-7430

    Enlarge

  44. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

    Enlarge

  46. pMN-334

    Plasmid
    #195468
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195468-plasmid-map-sequence-id-386007

    Enlarge

  50. mNeonGreen-Giantin

    Plasmid
    #98880
    Purpose
    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Giantin
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98880-plasmid-map-sequence-id-191166

    Enlarge

Showing: 8751 - 8800 of 157954 results