We narrowed to 21,608 results for: ESC

1. attB-GFP

   Plasmid

   #65521

   Purpose

   Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   attB-GFP
   

   Use

   Mouse Targeting; Bxb1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST-hPDI

   Plasmid

   #60555

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDI

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-agrBD-I

   Plasmid

   #53439

   Purpose

   T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7-lac

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSW002-PpsbA

   Plasmid

   #108236

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEBTet-SNAP-HCA_sensor

   Plasmid

   #127182

   Purpose

   Eukaryotic expression of the fusion protein used for sulfa-drugs sensing

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
   

   Use

   Tags

   HisTag and StrepTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRU51

   Plasmid

   #167677

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUB4-2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-NES-MBP-cATOM(Ras)

   Plasmid

   #209711

   Purpose

   Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   NES-MBP-cATOM(Ras)
   

   Use

   Tags

   NES-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-mIFP-P2A-GFP

   Plasmid

   #197168

   Purpose

   Expresses the protein of mIFP-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mIFP-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOVC1_EcSSPB-nano_X

   Plasmid

   #131212

   Purpose

   Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mEmerald-CENPB-N-22

    Plasmid

    #54037

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. SBP-mCherry-Ecadherin_puromycin

    Plasmid

    #65293

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mOrange2-H2B-6

    Plasmid

    #57962

    Purpose

    Localization: Nucleus/Histones, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGBW-m4046959

    Plasmid

    #145722

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046879

    Plasmid

    #149238

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMito.iGABASnFR.R205A

    Plasmid

    #112178

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R205A (no GABA binding)

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.CAG-FLEX.iGABASnFR.F102G

    Plasmid

    #112167

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mStrawberry-Vimentin-7

    Plasmid

    #55945

    Purpose

    Localization: Intermediate Filaments, Excitation: 574, Emission: 596

    Depositor

    Michael Davidson

    Article

    Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1 PIK3R1-Ni

    Plasmid

    #17744

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1-Ni (PIK3R1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Partial cDNA

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mRuby2-MannII-N-10

    Plasmid

    #55903

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MannII
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. ManII-SBP-EGFP_puromycin

    Plasmid

    #65260

    Purpose

    ManII reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated Mannosidae II
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGE8

    Plasmid

    #79463

    Purpose

    sgRNA shuttle vector; module 2E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGBW-m4046763

    Plasmid

    #145606

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046942

    Plasmid

    #145757

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. Str-KDEL_flGalT-SBP-tagBFP

    Plasmid

    #65274

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dendra2-Keratin-17

    Plasmid

    #57726

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1: TfR1-CoilE

    Plasmid

    #181988

    Purpose

    CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    TfR1-CoilE
    

    Use

    Tags

    CoilE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAL612

    Plasmid

    #119860

    Purpose

    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysterine 290 to Serine and Glycine 244 to Cystei…

    Promoter

    lac

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNC1136

    Plasmid

    #41562

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR-D-TOPO-PAmCherry1_160-236-MCS

    Plasmid

    #60546

    Purpose

    Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 C-terminal fragment
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    amino acids 160-236

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pENTR-D-TOPO-PAmCherry1_1-159-MCS

    Plasmid

    #60545

    Purpose

    Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 N-terminal fragment
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    amino acids 1-159

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKDR-TetOn.1

    Plasmid

    #183578

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKDR-TetOn.4

    Plasmid

    #183579

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKDR-EF1a.4

    Plasmid

    #183571

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKDR-EF1a.2

    Plasmid

    #183569

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Attenuator Sequence 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. px458_2A_GFP_sgRNA_NEMF

    Plasmid

    #127126

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA NEMF (NEMF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCEP-WAF1

    Plasmid

    #16450

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

43. GAE teo-GFP

    Plasmid

    #83958

    Purpose

    Can be used to produce inducible SIV-derived vector. Cells tranduced by lentiviral particles will overexpress GFP upon activation of the tetO promoter.

    Depositor

    Philippe Mangeot, Vincent Lotteau

    Article

    Mangeot et al Mol Ther. 2011 Sep;19(9):1656-66. doi: 1

    Insert

    TetO-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV.CAG-FLEX.iGABASnFR.F102Y.Y137L

    Plasmid

    #112168

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102Y.Y137L

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pGBW-m4046933

    Plasmid

    #145592

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. Lyn-FRB-GFP11

    Plasmid

    #181863

    Purpose

    Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    Lyn-FRB-GFP11
    

    Use

    Tags

    GFP11 and N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pGBW-m4136463

    Plasmid

    #149527

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge