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We narrowed to 9,305 results for: Pol

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Showing: 8851 - 8900 of 9305 results

  1. pMAL-c2X_MBP-LcgcN(C24S)-H6

    Plasmid
    #245527
    Purpose
    Maltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragment
    Depositor
    Henning Mootz
    Article
    Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202
    Insert
    N-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    C24S within the N-terminal fragment of the LCGC14…
    Promoter
    tac
    Available Since
    Nov. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    245527-plasmid-map-sequence-id-492806

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  11. pCJ1023

    Plasmid
    #228763
    Purpose
    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228763-plasmid-map-sequence-id-455783

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  13. pFB-D3-ASK1

    Plasmid
    #226619
    Purpose
    Co-expression of D3 and ASK1 complex in insect cells
    Depositor
    Ning Zheng
    Article
    Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:
    Inserts
    D3
    ASK1 (SKP1 Mustard Weed)
    Tags
    His tag followed with three copies of Msyb
    Expression
    Insect
    Mutation
    The flexible loop in D3 (Q478-D515) can be remove…
    Promoter
    Polyhedrin
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226619-plasmid-map-sequence-id-449175

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  19. pCB’ CsoS2 ΔNTD

    Plasmid
    #162705
    Purpose
    Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    pHnCB10 derived carboxysome operon, prk
    Expression
    Bacterial
    Mutation
    Deletion of residues 2-256 of CsoS2, Y131D
    Promoter
    PLtet0-1 promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162705-plasmid-map-sequence-id-341116

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  24. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana egg cell promoter
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

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  29. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid
    #60405
    Purpose
    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB2
    Expression
    Yeast
    Mutation
    TUB2 amino acids 429 - end, replaced with human T…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  45. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Promoter
    TEF1p
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

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  47. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  48. pCAG-hPPP3CA-FLAG

    Plasmid
    #179134
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179134-plasmid-map-sequence-id-352802

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Showing: 8851 - 8900 of 9305 results