We narrowed to 17,011 results for: Por

1. TRE-mRuby3

   Plasmid

   #214923

   Purpose

   expression vector control - contitutive expression of mRuby33 alone

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   mRuby3
   

   Use

   Lentiviral

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROSA26-Donor

   Plasmid

   #60952

   Purpose

   Porcine ROSA26 locus targeting

   Depositor

   Liangxue Lai

   Article

   Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

   Insert

   Porcine ROSA26 homologous ARM
   

   Use

   Gene targeting

   Tags

   Neo EGFP

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB.FRB-3myc-KLC1a

   Plasmid

   #134627

   Purpose

   Mammalian expression of mouse KLC1a

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KLC1a (Klc1 Mouse)
   

   Tags

   FRB, 3myc

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Chicken Beta actin

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT 3xFLAG-KIF20A

   Plasmid

   #192297

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KIF20A

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KIF20A (KIF20A Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-mGrn-myc

   Plasmid

   #178944

   Purpose

   Construct for expressing murine progranulin (Grn) in AAV

   Depositor

   Erik Roberson

   Article

   Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

   Insert

   Grn (Grn Mouse)
   

   Use

   AAV

   Tags

   Myc

   Promoter

   CBA

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-LaM4-3xTS

   Plasmid

   #162777

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXY349

   Plasmid

   #200262

   Purpose

   Expressing FtsW-TagRFP-T in bacterial cells

   Depositor

   Jie Xiao

   Article

   Yang et al Nat Microbiol. 2021 May;6(5):584-593. do

   Insert

   FtsW-tagRFP (ftsW )
   

   Tags

   tagRFP

   Expression

   Bacterial

   Promoter

   LacI

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA1 Flag ATXN1L

   Plasmid

   #33242

   Depositor

   Huda Zoghbi

   Article

   Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

   Insert

   ATXN1L (ATXN1L Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pBa.GFP-AP1/beta1

   Plasmid

   #218802

   Purpose

   Mammalian expression o fhuman AP1 b1

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   AP1 b1 (AP1B1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ROR1-V5/HIS

    Plasmid

    #205607

    Purpose

    Expression of human ROR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR1 receptor tyrosine kinase (ROR1 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+)-Caprola_14

    Plasmid

    #194675

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_14
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetro-XT-PAK2-CFP(puro)-L107F

    Plasmid

    #81180

    Purpose

    Retroviral vector for expression of kinase active PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Mutation

    L107F

    Promoter

    P-tight TRE

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

15. ZIKV_Trigger_32B

    Plasmid

    #75009

    Purpose

    Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_Trigger_32B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

    Plasmid

    #133560

    Purpose

    Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs_FLAG-SFXN5

    Plasmid

    #110639

    Purpose

    Retroviral expression of Flag-SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBXNPHM3-Nb_MsbA#1

    Plasmid

    #186428

    Purpose

    Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

    Depositor

    Markus Seeger

    Article

    Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

    Insert

    Nanobody Nb_MsbA#1
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3C cleavage site, His Tag, Maltose Binding Protei…

    Promoter

    pBAD

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEasyG3_mic

    Plasmid

    #184920

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FKBP12-CB2x2-mTagBFP2-HAx2

    Plasmid

    #207146

    Purpose

    Tandem calcium-buffer with rapalog dimerizer

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    Calbindin-2 (CALB2 Human)
    

    Tags

    FKBP12, HA, and mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24a-VHH-tev-APEX2

    Plasmid

    #117751

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB-Lmo2

    Plasmid

    #60666

    Purpose

    PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Lmo2 (Lmo2 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDisplay-GRAB_OTmut

    Plasmid

    #185385

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin control sensor GRAB_OTmut
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX458-sgRNA_Ago1_1

    Plasmid

    #73533

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX458-sgRNA_Ago1_2

    Plasmid

    #73534

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago1

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

28. Hy_pMT Flag-Hel25E WT

    Plasmid

    #110123

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPPC001

    Plasmid

    #171138

    Purpose

    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Sp.pCas9, BBa_J23107

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNHT7::RPL15::hGluc

    Plasmid

    #186768

    Purpose

    An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Gaussia luciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHisAlix deltaPRD

    Plasmid

    #42577

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    delta-PRD ALIX (residues 1-702) (PDCD6IP Human)
    

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

32. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89870

    Purpose

    expresses DRD2-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. XW17

    Plasmid

    #65835

    Purpose

    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Tags

    mCherry

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCI-TPI_PTC160-xrRNA-4H

    Plasmid

    #108369

    Purpose

    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EGFP-uTEV1-ACBD5

    Plasmid

    #171008

    Purpose

    HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-ACBD5(tmd)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFBP-4_1.sensor

    Plasmid

    #162844

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    4_1_RNA-device
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFBP-4_1m.mutant

    Plasmid

    #162869

    Purpose

    Yeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    4_1m_RNA-device
    

    Expression

    Yeast

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge