We narrowed to 16,114 results for: form

1. pPGK-T7/2-CD44v9 (-cyt)

   Plasmid

   #137819

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v9 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region

   Promoter

   PGK

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-hu-c-Myc-dN2

   Plasmid

   #50774

   Purpose

   retroviral expression of human c-Myc dN2 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN2 (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 42-439

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ncam1_0.0.0-Fc-His

   Plasmid

   #72086

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_0.0.0 (Ncam1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_ZKSCAN1_Tau7-12WT

   Plasmid

   #194166

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 7-12 WT
   

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ZKSCAN1_Tau10-12WT

   Plasmid

   #194163

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
   

   Tags

   3X flag tag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hu-c-Myc-dN1

   Plasmid

   #50773

   Purpose

   retroviral expression of human c-Myc dN1 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN1 (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 14-439

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLCHKO_TK1_HPRT1_Lb_1

   Plasmid

   #155059

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

   Plasmid

   #121816

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLPCX2-HA-GATA3 C1(259-443)

   Plasmid

   #89832

   Purpose

   Express GATA3 C1 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Gata3 (Gata3 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   C1 (AA 259-443)

   Promoter

   CMV

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-CMV GFP-LC3B G120

    Plasmid

    #123116

    Purpose

    Expresses EGFP-LC3B G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CFP-GIV-CT FA

    Plasmid

    #69533

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT-FA biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV w…

    Promoter

    CMV

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. human TLNRD1-F250D pET151

    Plasmid

    #159385

    Purpose

    Expresses human TLNRD1 F250D mutant in bacterial cells

    Depositor

    Ben Goult

    Article

    Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Changed Phenylalanine 250 to Aspartic Acid (F250D)

    Promoter

    T7

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKO_PDPR_exon_deletion_1

    Plasmid

    #155065

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLAP-BUBR1-dKARD

    Plasmid

    #114053

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

    Insert

    BUBR1-dKARD (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-CARMA2cl-FLAG

    Plasmid

    #97077

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CFP-GIV-CT WT

    Plasmid

    #69532

    Purpose

    Mammalian expression of The CFP-tagged GIV-CT biosensors

    Depositor

    Pradipta Ghosh

    Article

    Midde et al Proc Natl Acad Sci U S A. 2015 Mar 3;112

    Insert

    GIV (CCDC88A Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    C terminal amino acids S1660-S1870 of human GIV

    Promoter

    CMV

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1 hSlp2-a del.SHD

    Plasmid

    #40050

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a del.SHD (SYTL2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of SHD domain (1-56)

    Promoter

    CMV promoter

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Mouse, Synthetic)
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLCHKO_Ptbp_ex8_3

    Plasmid

    #155083

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGK-hMIGA2-DsRed

    Plasmid

    #192867

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLCHKO_SFRS7_exon_deletion_3

    Plasmid

    #155071

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLCHKO_MDM4_exon_deletion_3

    Plasmid

    #155075

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-C1 hSlp4-a del.SHD

    Plasmid

    #40036

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a del.SHD (SYTL4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted SHD domain (1-143)

    Promoter

    CMV promoter

    Available Since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

28. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid

    #121813

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMX-IY LC3B

    Plasmid

    #89291

    Purpose

    For expression of the mammalian Atg8 protein LC3B without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFUW-TetO-LITAF

    Plasmid

    #178444

    Purpose

    doxycycline-inducible expression of Human LITAF in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    LITAF (LITAF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Expression

    Mammalian

    Promoter

    RSV promoter

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMCh2007_pLenti_Syn_mChe-cdc42E7

    Plasmid

    #118620

    Purpose

    expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed cagtctg to ggcataa (667 - 673 nt in 3…

    Promoter

    synapsin I (rat)

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV_SEPT2 NCmut-mApple

    Plasmid

    #180321

    Purpose

    mammalian expression of human SEPT2 NCmut fused to monomeric Apple

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV_SEPT9_i1-i5-msfGFP

    Plasmid

    #180332

    Purpose

    mammalian expression of human SEPT9_i1-i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1-i5

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCHKU38.2-2.2

    Plasmid

    #110670

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    

    Expression

    Yeast

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV5-Flag-EPLIN beta delta LIM domain

    Plasmid

    #40938

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN beta delta LIM domain (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted

    Available Since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pEN_hU6miR-G12-E

    Plasmid

    #25757

    Purpose

    Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 12 miR-shRNA (Gna12 Mouse)
    

    Use

    Entry vector

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid

    #121800

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121819

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EGFP-Bin1b

    Plasmid

    #176014

    Purpose

    Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pEGFP-C1 hSlp4-a C2AB

    Plasmid

    #40037

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2AB (SYTL4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A and C2B domains (353-671)

    Promoter

    CMV promoter

    Available Since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPGK-T7/2-CD44v8-10 (-cyt)

    Plasmid

    #137822

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v8-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pEGFP-C1 hSlp4-a C2A

    Plasmid

    #40038

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2A (SYTL4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2A domain (353-487)

    Promoter

    CMV promoter

    Available Since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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44. FUW TetO NET1A-V5 WT

    Plasmid

    #69828

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Promoter

    minimal CMV promoter with tetracycline operator

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGL3-Basic-Fascin-Promoter (-402-0)

    Plasmid

    #89823

    Purpose

    Promoter luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Promoter

    SV40

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pEGFP-C1 hSlp4-a C2B

    Plasmid

    #40039

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a C2B (SYTL4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C2B domain (488-671)

    Promoter

    CMV promoter

    Available Since

    Oct. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMX-IY GBRPL2

    Plasmid

    #89295

    Purpose

    For expression of the mammalian Atg8 protein GABARAPL2 without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGEX4T-1 hSlp2-a del.C2AB

    Plasmid

    #40059

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a del.C2AB (SYTL2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of C2A and C2B domains (601-910)

    Promoter

    Tac

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

50. pTH744-CEN-RLuc/slowmaxCFLuc

    Plasmid

    #38224

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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