164,577 results

1. CD8a-EGFP

   Plasmid

   #86051

   Purpose

   A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CMV-KIF2C

   Plasmid

   #205994

   Purpose

   Over-expression of GFP-KIF2c

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   KIF2c (KIF2C Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF1684

   Plasmid

   #143965

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-3z/601

   Plasmid

   #26656

   Depositor

   Jonathan Widom

   Article

   Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

   Insert

   
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-ChrimsonR-tdT

   Plasmid

   #59171

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

   Plasmid

   #218860

   Purpose

   10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hGCG-ICAM(1235)-nrxn1b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmTurquoise2-Mito

   Plasmid

   #36208

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-CMV-human MYCN-EF1-mRFP

    Plasmid

    #193708

    Purpose

    Lentiviral expression of human MYCN

    Depositor

    Martine Roussel

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. H_NES 41kDa

    Plasmid

    #201346

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    HIV_NES-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. UAS-Syt::7xHalo7

    Plasmid

    #87650

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::7xHalo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::7xHalo7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW ymNeongreen-T2A-mCherry

    Plasmid

    #125700

    Purpose

    Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeongreen-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLXI_TRC401 SMARCB1 var 2

    Plasmid

    #111182

    Purpose

    Inducible vector (untagged) with SMARCB1 variant 2

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    BAF47 (SMARCB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PS6K-mcherry-iATPsnFR1.1

    Plasmid

    #227238

    Purpose

    Inducible ATP sensor for E. coli

    Depositor

    Fuzhong Zhang

    Article

    Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

    Insert

    iATPsnFR1.1
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. bactin2:loxP-STOP-loxP-DsRed-express

    Plasmid

    #68797

    Purpose

    For making transgenic fish

    Depositor

    David Traver

    Article

    Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

    Inserts

    beta-actin promoter
    loxp-stop-codon-loxp-dsred
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNCS mTangerine

    Plasmid

    #91763

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Tangerine
    

    Use

    Tags

    no tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlueBac human APC2

    Plasmid

    #11425

    Depositor

    Marc Kirschner

    Article

    Yu et al Science. 1998 Feb 20. 279(5354):1219-22.

    Insert

    APC2 (ANAPC2 Human)
    

    Use

    Baculoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pH2rU3_ForInd_mCherry_CMV_ZsGT2APurR

    Plasmid

    #204579

    Purpose

    lentivirus backbone cloning vector and template for barcoded non-neutralizable standard production

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR221_SLC8A1

    Plasmid

    #132162

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC8A1 (SLC8A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pACYCDuet-1:ChrA

    Plasmid

    #225079

    Purpose

    Expresses precursor peptide ChrA in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid

    #212022

    Purpose

    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GianCreg (GOLGB1 Human)
    

    Use

    Tags

    td5StayGold(c4)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-Moesin-N-14

    Plasmid

    #55104

    Purpose

    Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pUltra-U6-gRNAs1-5

    Plasmid

    #178569

    Purpose

    Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

    Depositor

    Christopher Lengner

    Article

    Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

    Insert

    5 gRNA-tRNA array with U6 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Human, Synthetic)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRK5F-TACE

    Plasmid

    #31713

    Purpose

    Mammalian expression of human TACE (ADAM17)

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TACE (ADAM17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pENTR TERRA

    Plasmid

    #111383

    Purpose

    TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    TERRA
    

    Use

    Gateway entry cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMC-1-ZmPLTP-6

    Plasmid

    #197713

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmPLTP promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and linked by IRES

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJG100

    Plasmid

    #138966

    Purpose

    Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge