4,143 results

1. pBK-VQR-BE3

   Plasmid

   #85171

   Purpose

   Expresses VQR-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VQR-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSicoR human Drosha1

   Plasmid

   #14766

   Depositor

   Tyler Jacks

   Article

   Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

   Insert

   Drosha shRNA (DROSHA Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pSLIK-Zeo

   Plasmid

   #25736

   Purpose

   Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pROSA26-PA

   Plasmid

   #21271

   Depositor

   Frank Costantini

   Article

   Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVPT-tTR-KRAB

   Plasmid

   #11642

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-p73α-pcDNA3

   Plasmid

   #22102

   Depositor

   William Kaelin

   Article

   Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

   Insert

   tumor protein p73 (TP73 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUW-tetO-hOCT4

   Plasmid

   #20726

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1098 pcDNA GFP FKHR

   Plasmid

   #9022

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   FKHR (FOXO1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LacZ in pLX304

   Plasmid

   #42560

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   2xV5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1/hChR2-EYFP

    Plasmid

    #20939

    Purpose

    Mammalian expression of humanized ChR2 fused to EYFP

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DsRed-rab9 WT

    Plasmid

    #12677

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223-JAK3

    Plasmid

    #23944

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223 SARS-CoV-2 NSP6

    Plasmid

    #141260

    Purpose

    Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51084

    Purpose

    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin1

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1-HyPer-C199S

    Plasmid

    #42213

    Purpose

    Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

    Depositor

    Vsevolod Belousov

    Article

    Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

    Insert

    HYPER C199S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nuclear EKAR (Cerulean-Venus)

    Plasmid

    #18681

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    monomeric Cerulean and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCVgfp::AID

    Plasmid

    #15925

    Depositor

    Nina Papavasiliou

    Article

    Dickerson et al J Exp Med. 2003 May 19. 197(10):1291-6.

    Insert

    AID (AICDA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-Brainbow-1.1 M

    Plasmid

    #18722

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVCT-rtTR-KRAB-2SM2

    Plasmid

    #11779

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge