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4,300 results

Showing: 3721 - 3740 of 4300 results

  1. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

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  4. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway Cloning
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141273-plasmid-map-sequence-id-288100

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  8. pEZ-Frt-lox-DT

    Plasmid
    #11736
    Purpose
    Conditional gene targeting vector; contain cloning sites for 5′ and 3′ homology arms, and a central targeting region flanked by LoxP sites, and a neomycin cassette flanked by FRT sites.
    Depositor
    Klaus Rajewsky
    Insert
    pEZ-Frt-lox-DT
    Use
    Cre/Lox; /frt
    Available Since
    May 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    11736-plasmid-map-sequence-id-7056

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Showing: 3721 - 3740 of 4300 results