159,141 results

1. mEos3.2-Tubulin-C-18

   Plasmid

   #57484

   Purpose

   Localization: Microtubules, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pMBIS

   Plasmid

   #17817

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

   Plasmid

   #134858

   Purpose

   Expresses EGFP-tagged early endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Ras-related protein 5A
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPR-v2-HygR-EGFP

   Plasmid

   #167188

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

   Plasmid

   #187952

   Purpose

   FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-CasRx-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker, HA-tag, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUW-tetO-lox-hSOX2

   Plasmid

   #20729

   Depositor

   Rudolf Jaenisch

   Article

   Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW005-2

   Plasmid

   #85611

   Purpose

   Empty gRNA delivery vector for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Rtn4a-GFP

    Plasmid

    #61807

    Purpose

    Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Rtn4a Stop Codon removed

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-dnMCAK

    Plasmid

    #205995

    Purpose

    inducible expression of HA-dnMCAK

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    dnMCAK
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pTight

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-PIK3C2A

    Plasmid

    #161988

    Purpose

    Mammalian expression vector containing PIK3C2A tagged with GFP

    Depositor

    Volker Haucke, David Bryant

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-hMeCP2-GFP

    Plasmid

    #48078

    Purpose

    Mammalian expression of GFP tagged MeCP2-WT

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pInducer-myc-ERK2-R67S/S153D

    Plasmid

    #213597

    Purpose

    Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R67S, S153D

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Frb-NLS-mCherry

    Plasmid

    #217613

    Purpose

    part B of SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    Frb-NLS-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_TRPM8

    Plasmid

    #64879

    Purpose

    Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

    Insert

    Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TFORF1066

    Plasmid

    #143919

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFATC2 (NFATC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG_Xph20-mNeonGreen-CCR5TC

    Plasmid

    #135534

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pD454-SR mouse Alpha-synuclein

    Plasmid

    #89075

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. MARIO/ pcDNA3

    Plasmid

    #124838

    Purpose

    mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

    Depositor

    Takeharu Nagai

    Article

    Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

    Insert

    MARIO
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. psPAX2-D64V-NC-COM

    Plasmid

    #131226

    Purpose

    Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    COM
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Dendra2-Farnesyl-5

    Plasmid

    #57717

    Purpose

    Localization: Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

25. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVoPo-GFP

    Plasmid

    #187840

    Purpose

    Constitutive GFP-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-Synapsin-pHoenix-WPRE

    Plasmid

    #70111

    Purpose

    lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
    

    Use

    Lentiviral

    Tags

    Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiCRISPRv2-STING_gRNA3

    Plasmid

    #127640

    Purpose

    Knock-out of human STING

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV INS2x mRFP-3MREs (AAV-beta)

    Plasmid

    #206928

    Purpose

    Beta cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Article

    Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INSx2

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hyeA3A-BE4max

    Plasmid

    #157944

    Purpose

    Mammalian expression of hyeA3A-BE4max

    Depositor

    Dali Li

    Article

    Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

    Insert

    hyeA3A-BE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    N57G

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-mEGFP-CaMKIIa (T286D)

    Plasmid

    #127391

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Aspartic acid

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mScarlet-Rab9a

    Plasmid

    #169074

    Purpose

    Express human Rab9a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab9a (RAB9A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3 RARE-RFP

    Plasmid

    #183054

    Purpose

    Fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFA6a-link-yoEGFP-Kan

    Plasmid

    #44900

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGRB

    Plasmid

    #71539

    Purpose

    for expression of gRNA in E. coli

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J231196

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMMLV-EGFP-P2A-Puro

    Plasmid

    #160226

    Purpose

    Expresses EGFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EGFP-P2A-PuroR
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRRL-MHCK7-GCaMP6

    Plasmid

    #65042

    Purpose

    Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

    Depositor

    Nenad Bursac

    Article

    Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

    Insert

    GCaMP6
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    MHCK7

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRK5-HA-Ubiquitin-K33

    Plasmid

    #17607

    Purpose

    Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K33 only, other lysines mutated to arginines; P19…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge