We narrowed to 13,665 results for: EGF

1. PX458-sgEIF4EBP1

   Plasmid

   #128097

   Purpose

   CRISPR gRNA against human EIF4EBP1 (4EBP1) with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   CRISPR sgRNA against human 4EBP1 (EIF4EBP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-FLT1-T777I

   Plasmid

   #116398

   Purpose

   Lentiviral expression of FLT1 T777I

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FLT1 (FLT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T777I

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. miniTol2 x EF1a_EKAREN4 + PGK-puro

   Plasmid

   #167824

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV x EF1a_EKAREN4(TA)-ires-puro

   Plasmid

   #167830

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEMS1401

   Plasmid

   #29198

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE-FLT4-F131S

   Plasmid

   #116401

   Purpose

   Lentiviral expression of FLT4 F131S

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   FLT4 (FLT4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   F131S

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

   Plasmid

   #167826

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. miniTol2 x EF1a_EKAREN4 + PGK-blast

   Plasmid

   #167825

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_EYS_p.N269H

    Plasmid

    #81557

    Purpose

    Gateway Donor vector containing EYS ,part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    N269H

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-FLEX-CAG_G-PTEN

    Plasmid

    #227438

    Purpose

    An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

    Depositor

    Tal Laviv

    Article

    Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

    Insert

    G-PTEN sensor (Pten Rat)
    

    Use

    AAV

    Tags

    CD-sREACh and mEGFP

    Expression

    Mammalian

    Mutation

    R14G

    Promoter

    pCAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-α-cateninV-

    Plasmid

    #229696

    Purpose

    Transient expression of GFP-alpha-cateninV- in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L344P

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pD649-HAsp-FLT1-COMP5AP-AviTag-9xHis

    Plasmid

    #157526

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-FLT4-COMP5AP-AviTag-9xHis

    Plasmid

    #157535

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLED.RAB

    Plasmid

    #193015

    Purpose

    Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP

    Plasmid

    #180795

    Purpose

    Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAecto&tm-PD-1int (BTLA, PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    SV40

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RG6-BAP1_E7-D192D

    Plasmid

    #154024

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    BAP1 c.576C>T, p.D192D

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RG6-BAP1_E11-G312G

    Plasmid

    #154026

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    BAP1 c.936T>G, p.G312G

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE-FLT1-G886V

    Plasmid

    #116394

    Purpose

    Lentiviral expression of FLT1 G886V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    G886V

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHAGE-FLT1-L778P

    Plasmid

    #116396

    Purpose

    Lentiviral expression of FLT1 L778P

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L778P

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-FLT1-R781Q

    Plasmid

    #116397

    Purpose

    Lentiviral expression of FLT1 R781Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R781Q

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-FLT1-V395I

    Plasmid

    #116399

    Purpose

    Lentiviral expression of FLT1 V395I

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    V395I

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-FLT1-W589*

    Plasmid

    #116400

    Purpose

    Lentiviral expression of FLT1 W589*

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    W589*

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223_EYS_p.F336L

    Plasmid

    #81582

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    F336L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223_EYS_p.H466N

    Plasmid

    #81517

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    H466N

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_EYS_p.R26Q

    Plasmid

    #81523

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R26Q

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_EYS_p.R587I

    Plasmid

    #81485

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R587I

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_EYS_p.C554S

    Plasmid

    #81491

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C554S

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223_EYS_p.Q172L

    Plasmid

    #81448

    Purpose

    Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EYS (EYS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Q172L

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEMS1132

    Plasmid

    #29053

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-Cre-NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEMS1215

    Plasmid

    #29109

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple97 (GPX3 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-Lamin-A

    Plasmid

    #206027

    Purpose

    Expresses GFP-tagged Lamin A in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Lamin-A (LMNA Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pD649-HAsp-KDR-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156967

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONR223-FLT4

    Plasmid

    #23923

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pD649-HAsp-FLT1-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156962

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pD649-HAsp-FLT4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156971

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    FLT4 (FLT4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDGB1alpha2_KanR_BastaR_Rb7_GFP

    Plasmid

    #186427

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    Rb7
    eGFP
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.1(+) Laccase2 MCS-mFndc3b

    Plasmid

    #126443

    Purpose

    Expresses mouse Fndc3b circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Calb1-T2A-dgCre targeting vector

    Plasmid

    #114438

    Purpose

    Target dgCre into the endogenous mouse Calb1 locus at the stop codon

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    dgCre
    

    Use

    Mouse Targeting

    Tags

    DHFR domain and EGFP

    Expression

    Mutation

    Promoter

    none; utilizes endogenous Calb1 promoter for expr…

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

    Plasmid

    #162878

    Purpose

    Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid

    #73215

    Purpose

    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Schistosoma japonicum, Human, Synthetic)
    

    Use

    Tags

    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …

    Expression

    Bacterial

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    T7

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pUt-mNF-BACH1

    Plasmid

    #199219

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSYC-201

    Plasmid

    #178963

    Purpose

    Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Inserts

    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    

    Use

    Tol2 mediated recombination

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    zebrafish gfap promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

    Plasmid

    #167832

    Purpose

    Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    HRAS(N17) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    Ubc, TRE

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDONR223-KDR

    Plasmid

    #23925

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    KDR (KDR Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    V297I

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDONR223-FLT1

    Plasmid

    #23912

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FLT1 (FLT1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S755P

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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50. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid

    #126444

    Purpose

    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge