We narrowed to 5,620 results for: pcas

1. pCAGG-NFIC2

   Plasmid

   #112701

   Purpose

   Expression of NFIC2 protein and GFP

   Depositor

   Richard Gronostajski

   Insert

   Nuclear Factor I C2 (Nfic Mouse)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-DsRed

   Plasmid

   #13771

   Purpose

   Flp recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   DsRed2
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGG-NFIX2

   Plasmid

   #112699

   Purpose

   Expression of NFIX2 protein and GFP

   Depositor

   Richard Gronostajski, Francois Guillemot

   Article

   Martynoga et al Genes Dev. 2013 Aug 15;27(16):1769-86. d

   Insert

   Nuclear Factor I X2 (Nfix Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-CBE6a

   Plasmid

   #215819

   Purpose

   Express CBE6a (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CyRFP1

   Plasmid

   #84356

   Purpose

   Expresses CyRFP1 fluorescent protein in an empty backbone, under CAG promoter

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   CyRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-NL1AB

   Plasmid

   #15262

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Neuroligin 1 with inserts A and B (Nlgn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9 TadCBEa

   Plasmid

   #193782

   Purpose

   Express TadCBEa (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa-SpCas9D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-T3-hCAS-pA

   Plasmid

   #48625

   Purpose

   Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii, Kunihiko Naito

   Article

   Fujii et al Nucleic Acids Res. 2013 Aug 30.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mammalian_SpCas9_Target-AID2S

    Plasmid

    #188648

    Purpose

    Expresses SpCas9_Target-AID2S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-delta90GFP

    Plasmid

    #26645

    Depositor

    Anjen Chenn

    Article

    Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

    Insert

    delta90 beta-catenin (Ctnnb1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of first 90 aa of beta catenin

    Promoter

    Available since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVNP2.0-SpCas9

    Plasmid

    #206880

    Purpose

    Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

    Insert

    SpCas9 (cas9 )
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-mGas8-PA

    Plasmid

    #176468

    Purpose

    Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    growth arrest specific 8 (Gas8 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

    Plasmid

    #198863

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human TERT gene

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    TERT sgRNA (TERT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. cacna2d1 HA pCAGGS

    Plasmid

    #206107

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549

    Depositor

    Annette Dolphin

    Article

    Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMA-SpCas9-g5

    Plasmid

    #80788

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-Voltron-ST

    Plasmid

    #119034

    Purpose

    Express soma-localized Voltron in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_eGCaMP(plus)

    Plasmid

    #201147

    Purpose

    expresses eGCaMP+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMA-SpCas9-g6

    Plasmid

    #80789

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-YTB

    Plasmid

    #26721

    Depositor

    Edward Callaway

    Article

    Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

    Insert

    venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS-N2c(G)

    Plasmid

    #73481

    Purpose

    N2c Glycoprotein expression vector

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    N2c(G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pX459_gRNA-AAVS1_hspCas9

    Plasmid

    #193309

    Purpose

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAH01Sp::Cas9D10A

    Plasmid

    #128161

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9D10A variant

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cav2.1 HA pCAGGS

    Plasmid

    #206076

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SpCas9-NG-CGBE1

    Plasmid

    #198537

    Purpose

    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-NCad-V

    Plasmid

    #191665

    Purpose

    mVenus-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-Cre

    Plasmid

    #125574

    Purpose

    Expresses Cre under the CAG promoter in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge