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Showing: 901 - 950 of 2174 results

  3. Tet-CA-Src-GFP

    Plasmid
    #83469
    Purpose
    Lentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cells
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Src (SRC Chicken)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Y527F (constitutively active)
    Promoter
    doxycycline-inducible promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83469-plasmid-map-sequence-id-159296

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  10. Tet-FRNK-GFP

    Plasmid
    #83472
    Purpose
    Lentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    FRNK (PTK2 Chicken)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    FRNK is the non-catalytic c-term domain of FAK ki…
    Promoter
    doxycycline-inducible promoter
    Available since
    Oct. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    83472-plasmid-map-sequence-id-159108

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  12. pENV8(GAAA)-GFPuv

    Plasmid
    #70128
    Purpose
    Negative control cobalamin riboswitch reporter vector for use in E. coli; deletion of regulatory kissing loop interaction by conversion of L5 to GAAA tetra loop
    Depositor
    Robert Batey
    Article
    Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:
    Insert
    kissing loop regulatory mutant of environmental cobalamin riboswitch sequence (env8)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70128-plasmid-map-sequence-id-130022

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  17. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  18. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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  19. pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)

    Plasmid
    #231234
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (Δ1-143), deletion of degron 1 & …
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231234-plasmid-map-sequence-id-459167

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  20. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  21. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154786-plasmid-map-sequence-id-306310

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  22. pCAGEN_NES_V5_GFP_FTA

    Plasmid
    #235633
    Purpose
    Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    GFP
    Use
    Tags
    FLAG-TEV cleavage site-AviTag and NES-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    235633-plasmid-map-sequence-id-467518

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  24. pCAG_EGFP-RandE3

    Plasmid
    #79870
    Purpose
    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-RandE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Beta-actin
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    79870-plasmid-map-sequence-id-149706

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  30. pAG415GPD-EGFP

    Plasmid
    #166137
    Purpose
    EGFP expression under the control of the GPD promoter yeast
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Insert
    EGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GDP
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166137-plasmid-map-sequence-id-328573

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  39. Dat - EGFP - Tol2

    Plasmid
    #85418
    Purpose
    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene
    Depositor
    Marc Ekker
    Article
    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:
    Insert
    dopamine transporter (slc6a3 Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    dat
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only

  46. pUASTLOTattB_mCherry-CD8-vhhGFP

    Plasmid
    #163930
    Purpose
    Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163930-plasmid-map-sequence-id-323460

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  48. PGK-GFP-LAMP1TM

    Plasmid
    #234874
    Purpose
    Lentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    LAMP-1 transmembrane domain and cytosolic tail
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234874-plasmid-map-sequence-id-469048

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Showing: 901 - 950 of 2174 results