We narrowed to 15,088 results for: pET

1. pPSV39-ugi

   Plasmid

   #186556

   Purpose

   Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   ugi
   

   Expression

   Bacterial

   Promoter

   LacUV5

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-PE

   Plasmid

   #162796

   Purpose

   All-in-one prime editor piggyBac transposon

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB-EPCP5

   Plasmid

   #228879

   Purpose

   Expression of Z-tagged LRRK2 KW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1846

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xNLS-NLP-cMyc-cMyc LbaCas12a

   Plasmid

   #182125

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc LbaCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/NDUFS2/myc-His

   Plasmid

   #127486

   Purpose

   Expresses myc-tagged NDUFS2 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS2 (NDUFS2 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAR100

   Plasmid

   #180377

   Purpose

   pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

   Depositor

   Alec Redwood

   Article

   Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

   Inserts

   NFAT-Luciferase
   hCD8A
   

   Expression

   Bacterial and Mammalian

   Promoter

   hEF1-HTLV and minimal promoter and an NFAT respon…

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/NDUFS7/myc-His

   Plasmid

   #127488

   Purpose

   Expresses myc-tagged NDUFS7 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS7 (NDUFS7 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSV39-dddAI

   Plasmid

   #186557

   Purpose

   Broad host range regulated expression of the DddA immunity determinant (DddAI)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-CMV-puro-2A-BLM

   Plasmid

   #127641

   Purpose

   Over-expression of human BLM

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   BLM (BLM Human)
   

   Use

   Lentiviral

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. hIL23p19 pEF6-V5 (MJS_004)

    Plasmid

    #72492

    Purpose

    Expression of human IL23p19 in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    IL23p19 (IL23A Human)
    

    Tags

    His/V5

    Expression

    Mammalian

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF019 MBP-β-catenin-His

    Plasmid

    #154070

    Purpose

    Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/NDUFV2/myc-His

    Plasmid

    #127491

    Purpose

    Expresses myc-tagged NDUFV2 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV2 (NDUFV2 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid

    #191093

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B and mScarlet
    

    Use

    AAV

    Tags

    H2B-V5-mScarlet

    Expression

    Mammalian

    Mutation

    The fusion protein was optimized to the Human cod…

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

    Plasmid

    #71216

    Purpose

    dCas9 fused to human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A catalytic domain
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3A

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid

    #133044

    Purpose

    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Tags

    NLS, TEV protease + TEV cleavage site, and miRFP6…

    Expression

    Mammalian

    Mutation

    S63K

    Promoter

    CAG

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. His6tev CBP BCHZ (1079-1751)

    Plasmid

    #99341

    Purpose

    Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
    

    Tags

    His6tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF073 MBP-Axin-His

    Plasmid

    #154011

    Purpose

    Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. H6SUMO-IntC-P53(304-393)

    Plasmid

    #200316

    Purpose

    bacterial expression of P53 TET-CTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Tags

    His6-SUMO and designed intein C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. H6GB1tevP53(1-303)IntN

    Plasmid

    #200312

    Purpose

    Bacterial expression of P53 NTAD-DBD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Tags

    His6GB1tev and designed intein N

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

    Plasmid

    #71217

    Purpose

    dCas9 fused to human DNMT3B catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3B catalytic domain
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDEST-mCherry-LacR-BRCA1 1366-1437

    Plasmid

    #71118

    Purpose

    mammalian expession of mCherry-LacR-BRCA1 1366-1437

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    contains amino acids 1366-1437 of BRCA1

    Promoter

    CMV/TO

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

    Plasmid

    #71218

    Purpose

    dCas9 fused to the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A(E752A)
    

    Use

    Lentiviral

    Tags

    SPdCas9-DNMT3A(E752A)

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid

    #221081

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NC15 pGLUE CUL3

    Plasmid

    #36970

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    CUL3 (CUL3 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pWPT-/mEGFP-1T-IRES-mCherry

    Plasmid

    #190190

    Purpose

    Lentiviral expression vector with altered Kozak sequence to direct EGFP expression. mCherry expression is regulated by an IRES.

    Depositor

    Alessandro Quattrone

    Article

    Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral

    Mutation

    changed EGFP Kozak sequence inserting a C>T mo…

    Promoter

    EIF1-short

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCALNL-mutKir2.1

    Plasmid

    #125579

    Purpose

    Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A non-conducting mutant (GYG -> AAA).

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FXYD2-luc

    Plasmid

    #31056

    Depositor

    Gerhart Ryffel

    Article

    Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

    Insert

    FXYD2 (FXYD2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    FXYD2 promoter from -2382 to -1

    Available Since

    Aug. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGlomyc-Klf4

    Plasmid

    #172870

    Purpose

    Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Klf4 (Klf4 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid

    #139457

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid

    #139458

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1/NDUFS8/myc-His

    Plasmid

    #127489

    Purpose

    Expresses myc-tagged NDUFS8 in mammalian cells

    Depositor

    Shashi Jain, Dieter Wolf

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS8 (NDUFS8 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pmiRGLO-miR-124-sponge (pos. CTRL)

    Plasmid

    #117327

    Purpose

    Dual luciferase assay positive control for miR-124 binding

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    miR-124 sponge
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRK5 Flag Grb10 5A short

    Plasmid

    #37484

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 5A (GRB10 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform C (missing N-term 58aa), 536aa, S150A T15…

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

    Plasmid

    #117326

    Purpose

    Dual luciferase assay negative control for miR-124 binding

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    reverse miR-124 sponge
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Murine PGK-1

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Lenti-EFS-hTSC1-T2A-Bsd

    Plasmid

    #193199

    Purpose

    Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LentiCRISPRCreb5gRNA

    Plasmid

    #195021

    Purpose

    Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    Creb5 gRNA (CREB5 Bovine)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMG058 MBP-GS 634-666-His

    Plasmid

    #154077

    Purpose

    Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GYS1 (GYS1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pRK5 Flag Grb10 9A long

    Plasmid

    #37485

    Depositor

    David Sabatini

    Article

    Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

    Insert

    Grb10 9A (GRB10 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    isoform A, 594aa, S104A S150A T155A S158A S426A S…

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

47. p4949 pLPCX-His-Xpress NLS hBrd4 CTD

    Plasmid

    #14459

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Retroviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

48. His6tev CBP BCHZDeltaAL

    Plasmid

    #99551

    Purpose

    Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
    

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    replaced 1557-1618 with SGGSG

    Promoter

    T7

    Available Since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. Lenti-sgTsc1#1/Cre

    Plasmid

    #173657

    Purpose

    Expresses a Tsc1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tsc1 (Tsc1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. Lenti-sgTsc1#2/Cre

    Plasmid

    #173658

    Purpose

    Expresses a Tsc1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Tsc1 (Tsc1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge