We narrowed to 10,444 results for: T7
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pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
UseTagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#133077PurposeX. laevis expression vector. It will generate the human TRAAK channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
UseTagsExpressionMutationQ258KPromoterT7Available sinceOct. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S100C
Plasmid#190010PurposeExpression of human TTR in E coliDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S100CPromotert7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Galectin-8 linker deletion
Plasmid#68806PurposeExpress Galectin-8 linker deletion protein in Bacterial cellsDepositorInsertGalectin-8 (LGALS8 Human)
UseTagsN-terminal 6-histidine-tagExpressionBacterialMutationNo mutationPromoterT7Available sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pRC/CMV
Plasmid#8514DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionMammalianMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI-elfn1_459to1199
Plasmid#87671PurposeIn situ probe for mouse Elfn1DepositorInsertElfn1 (Elfn1 Mouse)
UseTagsExpressionBacterialMutationpartial sequence, (459 to 1199bp off NM_175522.3)PromoterT7/T3Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA
Plasmid#60824Purposeto purify human ITPA from bacteriaDepositorInsertITPA (ITPA Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-eReader
Plasmid#135090PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered x3
UseTags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(515-710)-HinB
Plasmid#35053DepositorInsertIFI16-(515-710)-HinB (IFI16 Human)
UseTagsHisExpressionBacterialMutationContains HinB domain: aa 515-710PromoterT7Available sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
Syt2
Plasmid#186688PurposeExpresses Synaptotagmin 2 in E. ColiDepositorInsertSynaptotagmin 2
UseTagsGST and thrombin siteExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorTagsExpressionMammalianMutationPromoterTet responsive elementAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
V32M,L84M CoV-2 ORF8
Plasmid#159934PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
UseTags6x Histidine, TEV cleavableExpressionBacterialMutationV32M, L84MPromoterT7Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-7Zf-TYR
Plasmid#32786DepositorInsertTyrosinase (TYR Human)
UseIn vitro transcriptionTagsExpressionMutationI181MPromoterT7Available sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
C. difficile toxin B mRNA toehold switch sensor
Plasmid#110715PurposeToehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP outputDepositorInsertC. difficile toxin B toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28a His6Flag2AUF1(p42)
Plasmid#135941PurposeExpresses AUF1p42 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2 and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-P5CR
Plasmid#110295PurposeExpresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)DepositorInsertPyrroline-5-carboxylate Reductase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA
Plasmid#35052DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsHisExpressionBacterialMutationContains HinA domain: aa 192-393PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS1088
Plasmid#80159PurposepET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating EnzymeDepositorInsertHisSUMO-Cys-4xNuG2-Ald
UseTagsHisSUMOExpressionMutationPromoterT7Available sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
UseTagsGSTExpressionBacterialMutationT92APromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-ZZ(1700-1751)
Plasmid#99334PurposeBacterial expression of mouse CBP ZZ domainDepositorInsertCBP ZZ domain (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg2
Plasmid#170329PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 2 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationcodon optimizedPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET14b-mEKLF
Plasmid#67834PurposeEncodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitutionDepositorInsertEKLF (Klf1 Mouse)
UseTagsExpressionBacterialMutationFull-length mEKLF is aa 20-376; amino acid 19 is …PromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-Myr-FLAG-p110β-DM
Plasmid#55719Purposeeukaryotic expression of myristoylation tag (Myr-FLAG) p110b RBD double mutant S205D, K224ADepositorInsertp110 beta
UseTagsmyristoylation tag (Myr-FLAG)ExpressionMammalianMutationS205D, K224APromoterT7Available sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)
Plasmid#61939PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
UseTagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-At 14-3-3 phi
Plasmid#44939DepositorInsert14-3-3 phi (GF14 PHI Mustard Weed)
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND2_L17_S19_S11
Plasmid#132730PurposeT7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11DepositorInsert3WJ_NAND2_L17_S19_S11
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PorA-4xDQNAT
Plasmid#128392PurposeIn vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sitesDepositorInsertPorA
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only
