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169,371 results

Showing: 9551 - 9600 of 169371 results

  1. EBFP2-C1

    Plasmid
    #54665
    Purpose
    Localization: C1 Cloning Vector, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54665-plasmid-map-sequence-id-151383

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  4. pGM55

    Plasmid
    #71249
    Purpose
    luciferase reporter with hGM-CSF -55 to +28 minimal promoter
    Depositor
    Peter Cockerill
    Article
    Bert et al Plasmid. 2000 Sep;44(2):173-82.
    Insert
    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    GM-CSF minimal
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    71249-plasmid-map-sequence-id-131890

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  7. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  11. TFORF0144

    Plasmid
    #141536
    Purpose
    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PHOX2A (PHOX2A Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141536-plasmid-map-sequence-id-276744

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  12. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

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  14. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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  18. TFORF2801

    Plasmid
    #143621
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ETV2 (ETV2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    143621-plasmid-map-sequence-id-462950

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  19. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA(R118G)-HA
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Available Since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36047-plasmid-map-sequence-id-381279

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  20. LentiV_Neo_SIK3_CR_T221E

    Plasmid
    #108108
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108108-plasmid-map-sequence-id-207841

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  24. myc-tagged NRP1 (human)

    Plasmid
    #213068
    Purpose
    Express myc-NRP1 in mammalian cells
    Depositor
    Yoav Henis
    Insert
    neuropilin 1 (NRP1 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    N-terminal myc tag was introduced by a three-step…
    Promoter
    T7
    Available Since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213068-plasmid-map-sequence-id-421637

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  28. pJB158

    Plasmid
    #248751
    Purpose
    PopuA+20xTetO biosensor cicuit in pNBU2 integrative Bacteroides plasmid
    Depositor
    Carolina Tropini
    Insert
    PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-20xTetO-T7Te-rrnBT1-PopuA-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248751-plasmid-map-sequence-id-496751

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  29. pEM351

    Plasmid
    #248763
    Purpose
    80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-80xTetO
    Expression
    Bacterial
    Available Since
    Jan. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    248763-plasmid-map-sequence-id-496748

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  33. pEM414

    Plasmid
    #248766
    Purpose
    20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly
    Depositor
    Carolina Tropini
    Insert
    T7Te-rrnBT1-20xTetO
    Expression
    Bacterial
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    248766-plasmid-map-sequence-id-496747

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  34. tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry

    Plasmid
    #241314
    Purpose
    inducible mouse Adar Deaminase domain (HyperTRIBE) for expression in mammalian cells
    Depositor
    Stavros Lomvardas
    Insert
    Adar deaminase domain (Adar Mouse)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    E957Q
    Promoter
    tetO and CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    241314-plasmid-map-sequence-id-478721

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  38. TFORF1113

    Plasmid
    #143928
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RCOR3 (RCOR3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    143928-plasmid-map-sequence-id-471142

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  39. EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site

    Plasmid
    #228633
    Purpose
    Bacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A71 2A protease
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    C110A
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228633-plasmid-map-sequence-id-464103

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  40. PPE18-ALFA

    Plasmid
    #231247
    Purpose
    Expresses a C-terminally tagged PPE18 (ALFA tag)
    Depositor
    Bryan Bryson
    Insert
    PPE18-ALFA (PPE18 Mycobacterium tuberculosis)
    Tags
    ALFA Tag
    Expression
    Bacterial
    Promoter
    hsp60
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231247-plasmid-map-sequence-id-463614

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  42. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  43. TFORF0484

    Plasmid
    #143706
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFP92 (ZFP92 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143706-plasmid-map-sequence-id-409082

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Showing: 9551 - 9600 of 169371 results