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163,606 results

Showing: 9551 - 9600 of 163606 results

  4. PRE-Hsp70BbCas9_1.3

    Plasmid
    #190797
    Purpose
    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product
    Depositor
    Omar Akbari
    Article
    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024
    Insert
    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    190797-plasmid-map-sequence-id-376572

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  6. pLVX-EGFP-C1-centrin-1

    Plasmid
    #73331
    Purpose
    Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73331-plasmid-map-sequence-id-139044

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  8. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Article
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  9. CMV-mito-GEM-GECO1

    Plasmid
    #32461
    Purpose
    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    GEM-GECO
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…
    Promoter
    CMV
    Available Since
    Dec. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    32461-plasmid-map-sequence-id-123705

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  10. TFORF0139

    Plasmid
    #141533
    Purpose
    Lentiviral vector for overexpressing the UBTF transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    UBTF (UBTF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141533-plasmid-map-sequence-id-338772

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  11. pUltra-puro-RTTA3

    Plasmid
    #58750
    Purpose
    silence doxycyclin inducible pUltra-dox
    Depositor
    Yildirim Dogan, Kitai Kim
    Insert
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    loxP5171
    Promoter
    Ubiquitin C promoter
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    58750-plasmid-map-sequence-id-92492

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  14. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid
    #218863
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8m
    Use
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    A25G F286Y
    Promoter
    hsp70
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218863-plasmid-map-sequence-id-434232

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  18. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

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  33. pcDNA3.1-Clover-mRuby2

    Plasmid
    #49089
    Purpose
    Clover-mRuby2 tandem fusion for FRET calibration
    Depositor
    Kurt Beam
    Insert
    Clover-mRuby2 fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    49089-plasmid-map-sequence-id-114654

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  35. pGL4.22-VEGF-HRE::dLUC

    Plasmid
    #128096
    Purpose
    Hypoxia response element real-time luciferase reporter
    Depositor
    Chi Van Dang
    Article
    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:
    Insert
    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    Use
    Luciferase
    Tags
    drives expression of destabilized luciferase (2CP…
    Expression
    Mammalian
    Mutation
    Promoter
    inserted VEGF hypoxia response elements (HRE) (x5)
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128096-plasmid-map-sequence-id-249669

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  36. pAAVLINK-EF1alpha-5' IQGAP3

    Plasmid
    #236704
    Purpose
    5' AAVLINK plasmid for IQGAP3 expression
    Depositor
    Zhonghua Lu
    Insert
    IQGAP3 (IQGAP3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    Promoter
    EF1alpha
    Available Since
    Aug. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    236704-plasmid-map-sequence-id-480228

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  38. pUC19_CDC45_dTAG

    Plasmid
    #232733
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available Since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232733-plasmid-map-sequence-id-465446

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  39. pX459_MCM2_sgRNA

    Plasmid
    #232730
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232730-plasmid-map-sequence-id-465451

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  40. pX459_GINS4_gRNA

    Plasmid
    #232734
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232734-plasmid-map-sequence-id-465450

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  41. pX459_CDC45_sgRNA

    Plasmid
    #232732
    Purpose
    Generates CDC45 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    CDC45 (CDC45 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232732-plasmid-map-sequence-id-465449

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  42. pUC19_GINS4_dTAG

    Plasmid
    #232735
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232735-plasmid-map-sequence-id-465448

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  43. pUC19_MCM2_dTAG

    Plasmid
    #232731
    Purpose
    Generates MCM2 dTAG C ternimal knock-in cell lines as donor
    Depositor
    Junjie Chen
    Insert
    MCM2 (MCM2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Synonymous mutation
    Promoter
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232731-plasmid-map-sequence-id-465447

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  44. cacnb1b pCAGGS

    Plasmid
    #206102
    Purpose
    expression of rat beta1b calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacnb1b (Cacnb1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/B-actin
    Available Since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206102-plasmid-map-sequence-id-413014

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  46. pHR_PGK_SNIPR_CD8alpha variant 2, V319A

    Plasmid
    #188366
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD_V319A, a Notch1-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_V319A_Notch1-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188366-plasmid-map-sequence-id-371171

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  47. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid
    #100016
    Purpose
    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.
    Depositor
    Jeffery Miller
    Article
    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11
    Insert
    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SPTA1 erythroid specific promoter
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100016-plasmid-map-sequence-id-194507

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  48. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Use
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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Showing: 9551 - 9600 of 163606 results