Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,170 results

Showing: 9551 - 9600 of 177170 results

  3. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

    Enlarge

  6. CAG sHRPa-NRX

    Plasmid
    #73143
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-NRX
    Tags
    2x HA tag and Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73143-plasmid-map-sequence-id-134511

    Enlarge

  16. GPR120-Tango

    Plasmid
    #66310
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR120 (FFAR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    66310-plasmid-map-sequence-id-248122

    Enlarge

  19. pcDNA3-Cyto-CaNAR2

    Plasmid
    #64729
    Purpose
    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaNAR2-NES
    Tags
    Cerulean3, Nuclear export signal (NES), and YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    64729-plasmid-map-sequence-id-109343

    Enlarge

  20. GFP-TAF1

    Plasmid
    #65395
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TAF1 (TAF1 Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65395-plasmid-map-sequence-id-152041

    Enlarge

  23. pJZC116

    Plasmid
    #62344
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62344-plasmid-map-sequence-id-102267

    Enlarge

  24. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid
    #61888
    Purpose
    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61888-plasmid-map-sequence-id-101180

    Enlarge

  25. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62886-plasmid-map-sequence-id-105909

    Enlarge

  27. PX853

    Plasmid
    #62885
    Purpose
    N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa 2-573)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62885-plasmid-map-sequence-id-105908

    Enlarge

  31. Ratiometric FPX sensor for ERK kinase activity

    Plasmid
    #60974
    Purpose
    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A-WW domain-ERK substrate-ddFP B
    Tags
    ERK substrate is before ddFP copy B, WW domain is…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  32. FKBP_SCF2_LD6_NTEV

    Plasmid
    #58878
    Purpose
    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58878-plasmid-map-sequence-id-94239

    Enlarge

  34. pCAG-B19G

    Plasmid
    #59921
    Purpose
    Expression vector for Rabies virus SAD B19 glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus glycoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    59921-plasmid-map-sequence-id-94977

    Enlarge

  36. p201H Cas9

    Plasmid
    #59176
    Purpose
    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    hph
    Use
    CRISPR
    Promoter
    2x35S and StUbi-3P
    Available since
    Aug. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    59176-plasmid-map-sequence-id-120424

    Enlarge

  38. pUltra-dox

    Plasmid
    #58749
    Purpose
    doxycycline inducible multicistronic lentiviral gene expression system.
    Depositor
    Yildirim Dogan, Kitai Kim
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    TRE2 upstream of minimal CMV promoter
    Available since
    Aug. 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58749-plasmid-map-sequence-id-474986

    Enlarge

  40. AmCyan1-N1

    Plasmid
    #54616
    Purpose
    Localization: N1 Cloning Vector, Excitation: 453, Emission: 486
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    AmCyan1
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    54616-plasmid-map-sequence-id-244042

    Enlarge

  43. mEmerald-Fascin-C-10

    Plasmid
    #54094
    Purpose
    Localization: Actin Bundling Protein, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fascin (FSCN1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    3 Articles
Showing: 9551 - 9600 of 177170 results