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Showing: 9701 - 9750 of 17259 results

  23. pOPINB-hRNF216(511-784)

    Plasmid
    #171923
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171923-plasmid-map-sequence-id-346329

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  24. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid
    #68218
    Purpose
    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Luc / Renilla / P19
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos / 35S / 35S
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68218-plasmid-map-sequence-id-135582

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  26. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Available Since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  30. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

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  38. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid
    #123276
    Purpose
    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (BG505) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; SOSIP mutations; …
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    123276-plasmid-map-sequence-id-235240

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  50. pLenLox_U6 Nlgn2

    Plasmid
    #59358
    Purpose
    Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
    Depositor
    Peter Scheiffele
    Article
    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E
    Inserts
    Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Mouse, Rat)
    eGFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and mouse U6
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59358-plasmid-map-sequence-id-132852

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Showing: 9701 - 9750 of 17259 results