-
Plasmid#108456PurposeThis plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BACDepositorInsertloxP-myctagBFP-STOP-loxP-mCherry-NTR
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP
Plasmid#24033DepositorInsertNpr1 (npr-1 Nematode)
UseCre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP
Plasmid#195503PurposeSOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locusDepositorInsertSOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
UseCRISPR; Donor vectorTagsT2A-H2B-mCitrineExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xSTOP-lox (JDW 869)
Plasmid#224521PurposeA Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassetteDepositorInsertLoxP-3xStop-LoxP
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP
Plasmid#136064PurposeH2B-sfGFP cre reporterDepositorInsertHIST1H2BB (H2BC3 Human)
UseCre/LoxTagssfGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)ExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP
Plasmid#139485PurposePCR template for Halo-Tag gene knock-in donor vectorDepositorInsertHaloTag-loxP-P2A-dTKneo-pA-loxP
UseTagsHaloTagExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
bactin-loxp-BFP-loxp-dsred-pBSII
Plasmid#68972Purposefor transgenic animalsDepositorInsertslox-BFP-loxp
dsred
UseTagsExpressionMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mEGFP-loxP-P2A-dTKneo-pA-loxP
Plasmid#139484PurposePCR template for mEGFP gene knock-in donor vectorDepositorInsertmEGFP-loxP-P2A-dTKneo-pA-loxP
UseTagsmEGFPExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro
Plasmid#204720PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA
Plasmid#87584PurposePV5 for 3-input, 2-output Full AdderDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
Plasmid#35191DepositorInsertloxP KAN loxP 24xPP7SL MDN1
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-PuroR-LoxP
Plasmid#227502PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-NeoR-LoxP
Plasmid#227503PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α-loxP-GCaMP6f-loxP-WPRE
Plasmid#233288PurposeAAV vector with EF1α promoter and Cre-Out GCaMP6f, enabling to express GCaMP6f in Cre-negative cells.DepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox
Plasmid#196889PurposeDre-activated Cre-inactivated expression of lyn-mScarlet reporter.DepositorInsertlyn-mScarlet
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]
Plasmid#227245PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1α and pTRE3GAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA
Plasmid#113160PurposeA plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermU6Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE
Plasmid#32703DepositorTypeEmpty backboneUseCre/Lox and RetroviralTags3x HA tagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA
Plasmid#155125PurposeTol2 vector containing Cre-inducible QFGal4-switchDepositorInsertQFGal4-switch
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoterUbiAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP
Plasmid#32145DepositorInsertloxP> STOP STOP loxP>
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorInsertRFPnols-PQR-ZeocinR (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
Plasmid#35193DepositorInserttdtomato cyc loxP KAN loxP
UseTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)
Plasmid#129539Purposemodified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selectionDepositorTypeEmpty backboneUseCre/Lox; Mos1-mediated single copy insertion (mos…TagsExpressionWormMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
Plasmid#119064PurposeCre/lox flip-out construct for marking cell clones in Tribolium castaneumDepositorInsertspBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
Plasmid#227774PurposeMiddle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloningDepositorInsertloxP-dsR-loxP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204729PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1
Plasmid#196877PurposeNeuron-specific expression of mOrange2 reporterDepositorInsertmOrange2
UseCre/LoxTagsExpressionMammalianMutationPromoterQuimeric CAGAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTopo RE2 Donor pLox hPGK-Puro pA pLox
Plasmid#171049PurposeDonor template for CRISPR/Cas9 targeting of TERT RE2DepositorInsertPuromycin
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTopo RE3 Donor pLox hPGK-Puro pA pLox
Plasmid#171050PurposeDonor template for CRISPR/Cas9 targeting of TERT RE3DepositorInsertPuromycin
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron
Plasmid#172477PurposesfGFP with a synthetic intron derived from the human beta-globin geneDepositorInsertsfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-sfGFP+pCI syntron
Plasmid#172478PurposesfGFP with a synthetic intron from the pCI expression vector (Promega)DepositorInsertsfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron
Plasmid#172475PurposesfGFP with a synthetic intron derived from the mouse Ighe geneDepositorInsertsfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-sfGFP+RbG syntron
Plasmid#172476PurposesfGFP with a synthetic intron derived from the rabbit beta-globin geneDepositorInsertsfGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK
Plasmid#158616PurposeB6J Id1-LSL-DTA allele targeting vector, low copy numberDepositorInsertId1 (Id1 Mouse)
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationInsertion of IRES-loxP-NeoR-loxP-DTA after the st…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA
Plasmid#87580PurposePV1 for 3-input, 2-output Full AdderDepositorInsertNeomycin Resistance Gene
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
Plasmid#106171Purposecre mediated red to green shiftDepositorInsertsdsRED-express2
eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…PromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato
Plasmid#180152PurposetdTomato cre reporter with Rosa26 recombineering homology armsDepositorInserttdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-rox-STOP-rox-tRFP-pA-lox-ZsGreen-pA
Plasmid#175438Purposedual plasmid labelling cells of different neuronal population with different fluorophoresDepositorInsertrox-STOP-rox-tRFP-pA
UseCre/Lox; Dre/roxTagsZsGreen on backboneExpressionMammalianMutationPromoterpCAGAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)
Plasmid#85041PurposeThis plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.DepositorInsertloxP-stop-loxP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO
Plasmid#52419PurposeConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.DepositorInsertERAS (ERAS Human)
UseTagsExpressionMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectMutationPromoterAe. aegypti PUb and endogenous AGO1Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only