We narrowed to 13,937 results for: cas9

1. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Plasmid

   #101218

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant N692A/M694A/Q695A/H698A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A and H698A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

   Plasmid

   #208976

   Purpose

   Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   nSpCas9(H840)-BPNLS-P2A-GFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SpRY Cas9

   Plasmid

   #188491

   Purpose

   Expresses FLAG tagged SpRY Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCV-Cas9

   Plasmid

   #123643

   Purpose

   Expresses Cas9 with an N-terminal HUH-tag called PCV2

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   (EAAAK)8 linker and His6 + SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW-Cas9

   Plasmid

   #50661

   Purpose

   Doxycycline-inducible lentiviral expression of SpCas9

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   Tet ON

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Cas9-PCV

   Plasmid

   #123644

   Purpose

   Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Expression

   Plant

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Expression

   Plant

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   William Hahn, David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX311-Cas9

    Plasmid

    #118018

    Purpose

    Constitutive expression of Cas9

    Depositor

    William Hahn, David Root

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    Cas9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p201N Cas9

    Plasmid

    #59175

    Purpose

    Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

    Depositor

    Wayne Parrott

    Article

    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

    Inserts

    Cas9
    nptII
    

    Use

    CRISPR

    Promoter

    2x35S and StUbi-3P

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pM-Cas9

    Plasmid

    #196325

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    See depositor comments below

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cas9-GFP_sg_mAMPKa1

    Plasmid

    #79004

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa1 (Prkaa1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Cas9-GFP_sg_mAMPKa2

    Plasmid

    #79005

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    Prkaa2 (Prkaa2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCS2+ Cas9

    Plasmid

    #122948

    Purpose

    For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Promoter

    SP6

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge