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Plasmid#223557PurposePlasmid expressing GINIP-Nluc W139A in mammalian cellsDepositorInserthuman GINIP W139A - linker(GGGS) - Nluc
UseTags3xFLAGExpressionMammalianMutationW139A mutation in GINIP sequencePromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 WT
Plasmid#185632Purposeexpression of GST-tagged wild-type PML B-box1 domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 C4C4
Plasmid#185640Purposeexpression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationH155C H161CPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 W157E
Plasmid#185641Purposeexpression of GST-tagged mutant PML B-box1 W157E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationW157EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 F158E
Plasmid#185642Purposeexpression of GST-tagged mutant PML B-box1 F158E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationF158EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-MAFG-V5-miniTurbo
Plasmid#207818PurposeC-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertMAFG-V5-miniTurbo
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-MAFG
Plasmid#207817PurposeN-terminal tagged MAFG for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-MAFG
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ZNF746
Plasmid#207819PurposeN-terminal tagged ZNF746 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-ZNF746 (ZNF746 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-A
Plasmid#207823PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-NS-B
Plasmid#207824PurposeDual expression of Cas9 and non-specific sgRNADepositorInsertnon-specific sgRNA
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-ZNF746-C571A
Plasmid#207826PurposeDox-inducible expression of ZNF746-C571ADepositorInsertZNF746-C571A (ZNF746 Human)
UseLentiviralTagsExpressionMutationC571APromoterTREAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ZNF746-V5-miniTurbo
Plasmid#207820PurposeC-terminal tagged ZNF746 for miniTurbo proximity experimentsDepositorInsertZNF746-V5-miniTurbo (ZNF746 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATo
Plasmid#203746PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrLATo (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
UseTags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-Y-L
Plasmid#153546Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucineDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsExpressionMammalianMutationwith 6 Y in 3 ITAMs mutated to LPromoterCMVAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-EGFP
Plasmid#86059PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
UseTagsGFPExpressionMammalianMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-cystin-CTS
Plasmid#86066PurposeA bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.DepositorInsertCystin (Cys1 Mouse)
UseTagsGSTExpressionBacterialMutationcontains the region from 28-35PromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFFΔC-Myc
Plasmid#86060PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.DepositorInsertFibrocystin (Pkhd1 Mouse)
UseTagsMycExpressionBacterialMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P50L
Plasmid#60533PurposeDonor vector for human Pak5 P50L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP50LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P76T
Plasmid#60534PurposeDonor vector for human Pak5 P76T mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP76TPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S312C
Plasmid#60535PurposeDonor vector for human Pak5 S312C mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS312CPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V463L
Plasmid#60536PurposeDonor vector for human Pak5 V463L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV463LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V593I
Plasmid#60539PurposeDonor vector for human Pak5 V593I mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV593IPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-470)
Plasmid#51724Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 471-660PromoterAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(471-660)
Plasmid#51766Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 1-470PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7Jip1(1-126)
Plasmid#51702Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 127-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 Jip1(1-281)
Plasmid#51700Purpose" JIP-1 may suppress signal transduction by the JNK pathway"DepositorInsertJnk interacting protein 1 (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted 282-660PromoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationI250 (silent mutation in YFP)PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc
Plasmid#223559PurposeAlias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotorDepositorInsertYFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K
Plasmid#110160PurposeExpression of human eEF2K in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cx36-GCaMP
Plasmid#123604PurposeMouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cellsDepositorInsertConnexin 36-GCaMP3 fusion (Gjd2 Mouse)
UseTagsGCaMP3ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-LAR-GECO1.2
Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-SBFP2
Plasmid#187964PurposeMammalian cell expression of a fluorescent SBFP2-tagged mitochondrial markerDepositorInsertMito (COX8A Human)
UseTagsSBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-Mart1-SABR
Plasmid#119052Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBIDepositorInsertA0201-Mart1-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NRF1-V5-miniTurbo
Plasmid#207810PurposeC-terminal tagged NRF1 for miniTurbo proximity experimentsDepositorInsertNRF1-V5-miniTurbo (NFE2L1 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-NRF1
Plasmid#207809PurposeN-terminal tagged NRF1 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-NRF1 (NFE2L1 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-CanlonicSF
Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
UseTagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pg-EGFP-HIF-2alpha
Plasmid#87207PurposeN-terminally EGFP-tagged human HIF-2a/EPASDepositorInsertHIF-2a (EPAS1 Human)
UseTagsEGFPExpressionMammalianMutationQ868EPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-FGFR4
Plasmid#60531PurposeEntry vector for human FGFR4DepositorInsertFGFR4 (FGFR4 Human)
UseEntry vectorTagsExpressionMutationc2134a P712TPromoterNAAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-SABR-Backbone
Plasmid#119051Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-KK10-SABR
Plasmid#119053Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBIDepositorInsertB2705-KK10-SABR
UseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHLdeltaB-IRES-GFP
Plasmid#187642PurposeMutant VHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationdeletion of amino acids 91 to 121PromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL113
Plasmid#22471PurposeExpresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.DepositorInsertPhyB NT (PHYB Mustard Weed, Mouse)
UseSynthetic BiologyTags10aaLinker-mCFP-HrasCTExpressionMammalianMutationY276HPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAL130
Plasmid#22475PurposeExpresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to PhyDepositorInsertPIF3NT (PIF3 Mustard Weed, Mouse)
UseSynthetic BiologyTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-2P
Plasmid#203748PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27AC30A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27A, C30APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Tlr4(Lps)
Plasmid#27149DepositorInserttoll-like receptor 4(Lps) (Tlr4 Mouse)
UseTags3x FLAGExpressionMammalianMutationchanged Proline 712 to Histidine. Native signal p…PromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only