165,065 results

1. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWZL Neo Myr Flag YES1

   Plasmid

   #20665

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   YES1 (YES1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

   Plasmid

   #107943

   Purpose

   Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
   

   Tags

   3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmTurquoise2-Drp1

   Plasmid

   #110946

   Purpose

   Expresses dynamin-like protein 1 fused to mTurquoise2

   Depositor

   Pablo Rivera-Fuentes

   Insert

   Dynamin-1-like protein (DNM1L Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mNeonGreen-JIP4

   Plasmid

   #164621

   Purpose

   Expresses JIP4 in mammalian cells with a mNeonGreen tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP4 (SPAG9 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dead Sa Cas9

   Plasmid

   #138162

   Purpose

   For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   dSaCas9
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D10A + N580A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HBx

   Plasmid

   #65463

   Purpose

   expresses HBx in mammalian cells

   Depositor

   Wang-Shick Ryu

   Article

   Cha et al Hepatology. 2004 Jun;39(6):1683-93.

   Insert

   HBV X protein
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSET-MagLOV

   Plasmid

   #219957

   Purpose

   Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

   Depositor

   Andrew York

   Article

   Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

   Insert

   MagLOV
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   AsLOV2 C450P L496V Q513K G528K D540M

   Promoter

   T7

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCitrine-pBAD

    Plasmid

    #54723

    Purpose

    Localization: Protein Expression Vector , Excitation: 516, Emission: 529

    Depositor

    Oliver Griesbeck, Roger Tsien, Michael Davidson, Robert Campbell

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Type

    Empty backbone

    Tags

    6xHis and mCitrine

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M-SPOTIT1.1

    Plasmid

    #171216

    Purpose

    Expresses a mu-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    M-SPOTIT1.1
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Expression

    Bacterial

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TALEN-CCR5L18-CtermQ3

    Plasmid

    #51440

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) Q3 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K788Q, R792Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TALEN-CCR5R18-CtermQ3

    Plasmid

    #51441

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) Q3 C-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K788Q, R792Q and R801Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3 Flag beta-1-adrenergic-receptor

    Plasmid

    #14698

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B1AR (ADRB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-Fis1

    Plasmid

    #182580

    Purpose

    Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Fis1
    

    Tags

    Fis1

    Expression

    Mammalian

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid

    #133732

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Promoter

    pCamk2a and pTRE3G-Bi

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry KNL1 wt hardened

    Plasmid

    #45224

    Depositor

    Michael Lampson, Iain Cheeseman

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
    

    Tags

    TEV S15

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

23. iCAP-ROOT

    Plasmid

    #192262

    Purpose

    AAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid

    Depositor

    Li Ye

    Article

    Wang et al Nature. 2022 Sep;609(7927):569-574. doi:

    Insert

    Synthetic construct for ROOT VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMK198 (GAL-OsTIR1)

    Plasmid

    #140655

    Purpose

    GAL-OsTIR1(WT)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT)
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-AAVS1-Ef1a-Blast

    Plasmid

    #227267

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC13N-dCas9-BFP-KRAB

    Plasmid

    #127968

    Purpose

    constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-dCas9-NLS-BFP-KRAB
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSBbi-pur H-2Kb

    Plasmid

    #111623

    Purpose

    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

    Depositor

    Jon Yewdell

    Insert

    H2Kb
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Expression

    Yeast

    Available Since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge