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167,150 results

Showing: 10201 - 10250 of 167150 results

  2. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  4. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  16. pcDNA3-Flag-RNF5

    Plasmid
    #122043
    Purpose
    express RNF5
    Depositor
    Ze'ev Ronai
    Insert
    RNF5 (RNF5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122043-plasmid-map-sequence-id-242455

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  22. mEmerald-Alpha1-Catenin-C-18

    Plasmid
    #53982
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Alpha1-Catenin
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. lox2272 Neo* lox2272

    Plasmid
    #168769
    Purpose
    lox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168769-plasmid-map-sequence-id-337468

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  26. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  31. pMXS-mitoGshF-blasticidin

    Plasmid
    #210314
    Purpose
    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210314-plasmid-map-sequence-id-418848

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  41. IronFistMUT.

    Plasmid
    #243008
    Purpose
    Iron binding deficient IronFist
    Depositor
    Roland Malli
    Article
    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d
    Inserts
    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    Use
    Gateway
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    H15A, H57A, E58A, E61A, H126A, E130A
    Promoter
    human phosphoglycerate kinase (hPGK)
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    243008-plasmid-map-sequence-id-486388

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  45. pcDNA3.1-Beta1

    Plasmid
    #140987
    Purpose
    Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GBeta1 (GNB1 Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal human rhinovirus (HRV) 3C …
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140987-plasmid-map-sequence-id-301588

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  48. pAttBFHyg

    Plasmid
    #244926
    Purpose
    attB Hyg Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244926-plasmid-map-sequence-id-486007

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Showing: 10201 - 10250 of 167150 results