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We narrowed to 18,759 results for: nls

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Showing: 8281 - 8320 of 18759 results

  4. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112922
    Purpose
    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Gateway Cloning
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112922-plasmid-map-sequence-id-217731

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  6. dCas9 gateway compatible

    Plasmid
    #128322
    Purpose
    dCas9 C-terminal gateway cloning vector
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128322-plasmid-map-sequence-id-255033

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  7. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Publication
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118992-plasmid-map-sequence-id-232118

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  8. pMTG07-Amp-FRT

    Plasmid
    #128276
    Purpose
    Gene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65
    Depositor
    Gabor Balazsi
    Publication
    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gal4-VVD-p65
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128276-plasmid-map-sequence-id-251841

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  15. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  19. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Publication
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  24. pCDNA3.1-H-Luc_nucl

    Plasmid
    #113931
    Purpose
    Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET
    Depositor
    Kai Johnsson
    Publication
    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H-Luc_nucl
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    113931-plasmid-map-sequence-id-222136

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  28. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102890
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102890-plasmid-map-sequence-id-198249

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  29. pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB

    Plasmid
    #111504
    Purpose
    Expresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Publication
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, S122R, a…
    Promoter
    CMV
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111504-plasmid-map-sequence-id-214283

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  32. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Publication
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109399-plasmid-map-sequence-id-211963

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  34. pCMV-dCpf1-BE-YEE

    Plasmid
    #107687
    Purpose
    Expresses dLbCpf1-BE-YEE in mammalian cells
    Depositor
    Jia Chen
    Publication
    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1-BE-YEE
    Expression
    Mammalian
    Mutation
    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…
    Promoter
    CMV
    Available since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    107687-plasmid-map-sequence-id-207602

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  36. pX551-miniCMV-AsCpf1

    Plasmid
    #107034
    Purpose
    AAV plasmid expressing AsCpf1 under control of miniCMV promoter
    Depositor
    Alex Hewitt
    Insert
    AsCpf1
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107034-plasmid-map-sequence-id-208553

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  38. pUASTattb-cAMPr Y205A, R626A

    Plasmid
    #84932
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
    Depositor
    Justin Blau
    Publication
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R626A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R626A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84932-plasmid-map-sequence-id-180302

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Showing: 8281 - 8320 of 18759 results