We narrowed to 10,863 results for: HA
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Plasmid#191411PurposeTransfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).DepositorInsertBcl-2-interacting killer (BIK Human)
UseTagsVenusExpressionMammalianMutationwith deletion of amino acids 72-136 (del72-136) i…PromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HySp5-227
Plasmid#192996PurposePlasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domainDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP box and DNA binding domainPromoterAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5-337
Plasmid#192994PurposePlasmid that encodes for Hydra Sp5, lacking the DNA binding domainDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno DNA binding domainPromoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-HA.HA.HA.Mm.RAP1
Plasmid#184646PurposeMammalian expression of mouse Rap1DepositorInsertrap1
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable SinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH2exp
Plasmid#131019PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1ec
Plasmid#131020PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MNEAP1exp
Plasmid#131021PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMNEAP1
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MKAKU41ec
Plasmid#131014PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
MKAKU41exp
Plasmid#131015PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMKAKU41
UseTagsmCherry-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NCH1ec
Plasmid#131016PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH1exp
Plasmid#131017PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH1
UseTagseGFP-FLAG-HAExpressionPlantMutationPromoterP35SAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
NCH2ec
Plasmid#131018PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertNCH2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable SinceMay 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1α -NWM-Node 1
Plasmid#154253Purposeexpresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cellsDepositorInsertreconstructed Node 1 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 2
Plasmid#154254Purposeexpresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cellsDepositorInsertreconstructed Node 2 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1α -NWM-Node 3
Plasmid#154255Purposeexpresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cellsDepositorInsertreconstructed Node 3 of New World monkey retroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCsB_spacer
Plasmid#136076PurposeUse when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCsA_spacer
Plasmid#136075PurposeUse when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
OfCasp3b
Plasmid#166054Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertOrbicella faveolata caspase-3b
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PaCasp3
Plasmid#166052Purposea caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6DepositorInsertPorites astreiodes caspase-3
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046858
Plasmid#145658PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046730
Plasmid#145659PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BII-Sh-PnGW-CMV
Plasmid#133366PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-eSpCas9
Plasmid#133381PurposePiggybac vector for constitutive expressionDepositorInserteSpCas9
UseTagsHAExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV-Luc
Plasmid#133367PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W-Luc
Plasmid#133362PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W
Plasmid#133361PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-Luc
Plasmid#133360PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR 1-55 eGFP SV40
Plasmid#132651PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 55 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-ATG13[3SD](426)
Plasmid#122077PurposeExpressing HA-ATG13[3SD]DepositorInsertATG13
UseTags3HAExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-SpAtg17(416)
Plasmid#122079PurposeExpressing S. pombe HA-Atg17DepositorAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A R181A R182A R186A (N4A)
Plasmid#111940PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutationDepositorInsertHsh155 T178A R181A R182A R186A (N4A)
UseTagsExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A R775A R776A Q778A (C4A)
Plasmid#111946PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutationDepositorInsertHsh155 Q773A R775A R776A Q778A (C4A)
UseTagsExpressionBacterial and YeastMutationQ773A R775A R776A Q778APromoterAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp1
Plasmid#69196Purposecomplementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.DepositorInsertTRP1
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38Q
Plasmid#65094PurposeDestination vector with RBPMS with R38Q mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged arginine 38 to glutaminePromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-F2-GCA
Plasmid#30720DepositorInsertF2 GCA
UseMrna productionTagsHAExpressionMutationPromoterAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2-F2-GCG
Plasmid#30722DepositorInsertF2 GCG
UseMrna productionTagsHAExpressionMutationPromoterAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only