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We narrowed to 6,995 results for: tet

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Showing: 1001 - 1050 of 6995 results

  1. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  2. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  5. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  7. pKAR1/Pur

    Plasmid
    #23105
    Purpose
    Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid. Contains puromycin resistance gene
    Depositor
    Randy Poon
    Article
    Ma et al Nucleic Acids Res. 2007 . 35(4):e22.
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    TRE for cDNA; U6 for shRNA
    Available Since
    May 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pTet-O-APOE-E3-T2A-Puro

    Plasmid
    #190812
    Purpose
    Express APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Mutation
    E3
    Promoter
    Tet-O
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190812-plasmid-map-sequence-id-372860

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  21. pTet-O-APOE-E2-T2A-Puro

    Plasmid
    #190811
    Purpose
    Express APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance gene
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Mutation
    E2
    Promoter
    Tet-O
    Available Since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190811-plasmid-map-sequence-id-372859

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  32. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71428-plasmid-map-sequence-id-440608

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  35. pCS2-UTX-F-MT2

    Plasmid
    #40619
    Purpose
    Expression of enzyme-dead UTX in mammalian cells
    Depositor
    Kai Ge
    Article
    Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.
    Insert
    UTX (KDM6A Human)
    Tags
    6xMyc and Flag
    Expression
    Mammalian
    Mutation
    changed Histidine 1146 and Glutamic Acid 1148 to …
    Promoter
    CMV
    Available Since
    Nov. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  38. pBI-MCS-EYFP

    Plasmid
    #58855
    Purpose
    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    enhanced yellow fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58855-plasmid-map-sequence-id-98989

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  40. pBB328

    Plasmid
    #221533
    Purpose
    Plasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.
    Depositor
    Brett Barney
    Inserts
    vioABCE
    Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
    Tetracycline antibiotic cassette from pRK415
    Expression
    Bacterial
    Promoter
    None, contains RBS site
    Available Since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    221533-plasmid-map-sequence-id-442457

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  43. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available Since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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  50. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181946-plasmid-map-sequence-id-356600

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Showing: 1001 - 1050 of 6995 results