We narrowed to 14,145 results for: TIM
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Plasmid#51638PurposeExpresses Cas9-nickase in mammalian cells and zygotes.DepositorInsertCodon-optimized Cas9 D10A
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianPromoterCAG promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLPS2
Plasmid#209972PurposeContains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmidsDepositorInsertRBS (Lp)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD2529-CAG-Flag-mouse TGFb3
Plasmid#180262PurposeMouse full length TGFβ3 expressionDepositorAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290
Plasmid#129670PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneExpressionPlantMutationAacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PIP2-CFP(ST) sensor
Plasmid#170355PurposeCFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.DepositorAvailable SinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS tat-P2A-rev
Plasmid#101331PurposeLentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)DepositorInsertNL4-3 tat and rev
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
M-SPn-VP64
Plasmid#48674PurposeMammalian SP-VP64 nuclease-null Cas9 activator expression, human optimizedDepositorInsertCas9-VP64, nuclease-null
UseCRISPRTagsNLS and VP64Mutationnuclease-nullPromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
MX01
Plasmid#158624PurposeGATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).DepositorTypeEmpty backboneUseGateway destination vector ; bacmam acceptor vect…TagsP2AExpressionMammalianPromoterCMV-IEAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p119 (FUGW-UbCp-rtTA3)
Plasmid#105171Purposeubiquitin C promoter drives rtTA3 expressionDepositorInsertUbCp-rtTA3
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLAG-SIV-RCM-vpx
Plasmid#115844PurposeEncodes codon optimized FLAG tagged SIVRCM-VpxDepositorInsertSIVRCM-Vpx
Tags3x-FLAGExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mVenus-I
Plasmid#163763PurposeExpresses Streptomyces codon-optimised mVenus-IDepositorInsertmVenus-I
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterSP44Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_ANP
Plasmid#133243PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertrat atrial natriuretic peptide-fused codon-optimized GCaMP6s
ExpressionInsectPromoterhsp70Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292
Plasmid#129672PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationAaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1149 - intron 3 smu-2 PATCs
Plasmid#161516PurposePATC-rich intron number three from smu-2 to stimulate germline expression in C. elegansDepositorInsertIntron 3 from smu-2
ExpressionWormAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN599 (pFuGW-hUbCp-hSTE1)
Plasmid#105206Purposeubiquitin C promoter drives STE expressionDepositorInserthUbCp-hSTE1
UseLentiviral and Synthetic BiologyTagsHA tagExpressionMammalianAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
JH04
Plasmid#73513PurposemRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2DepositorInsertpmex-5::mRuby2::PH::tbb-2
TagsmRuby2Promotermex-5Available SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-efSaCas9-BlastR
Plasmid#220971PurposeExpresses human codon-optimized efSaCas9DepositorInsertefSaCas9
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymVenus-URA3
Plasmid#168058PurposeReplaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymVenus
TagsymVenusExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR40
Plasmid#69257Purposeplin-31::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterlin-31Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialPromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
ExpressionBacterialAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CH00X
Plasmid#73517PurposeTagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-TDepositorInsertpmex-5::TagRFP-T::PH::tbb-2
TagsTagRFP-TPromotermes-5Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-EEA-g1-PGK-Puro
Plasmid#102908PurposePiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-guide1-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
ExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-L1PA8CHmneo
Plasmid#69608PurposeExpresses a neomycin tagged codon optimized L1PA8DepositorInsertL1PA8
Tagsmneo retrotransposition cassetteExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
TOPO rtTA3-2A-Cre
Plasmid#68448PurposeTOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible geneDepositorInsertrtTA3-2A-Cre
UseGmapExpressionBacterialMutationCodon optimized for expression in micePromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_1
Plasmid#73525PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRExpressionMammalianPromoterhU6Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCas13 Twin-SUMO
Plasmid#199753PurposeExpresses codon optimized miniature mCas13 for protein expression in E.coliDepositorInsertmCas13
TagsSUMOExpressionBacterialPromoterLacIAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETCON-TNF
Plasmid#67748PurposeTumor Necrosis Factor Alpha Yeast Display PlasmidDepositorInsertTNF-Alpha
ExpressionBacterial and YeastAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term 3C eGFP myc
Plasmid#160685PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneTagsHis8 eGFP 3CExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNC1136
Plasmid#41562DepositorInsertUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
MutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
Integrin alpha5(1-623)-AHCys
Plasmid#27301DepositorInsertintegrin alpha 5 (ITGA5 Human)
ExpressionMammalianMutationalpha 5(1-623)+GG+TEV+GGEN+AHCysAvailable SinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH760-CEN-mCherry_v3
Plasmid#40598DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
ExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Blat-BSD
Plasmid#163791PurposeThe plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.DepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - DDX3Y sgRNA 2
Plasmid#70657PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against DDX3Y
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
Plasmid#31478DepositorInsertSFFV HA NLS Y2 K227M T2A BFP
UseLentiviralTagsHA and NLSMutationF13Y, S111Y, K227M = Y2 nickase "reo" …Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSR33
Plasmid#69254Purposephsp16.2::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.2Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
human b1 ecto-pIRES
Plasmid#113387PurposeExpress human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal claspDepositorInserthuman beta 1 ectodomain
TagsHis tagExpressionMammalianPromoterCMVAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only