We narrowed to 18,651 results for: Met
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Plasmid#123155PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Ares-Aphrodite
Plasmid#13571DepositorInsertFLIPglu F16A Delta11 Aphrodite
TagsAphrodite and AresExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD_NLS
Plasmid#124625PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLSDepositorInsertMCP_ADAR1 DD_NLS
UseAAVTagsNLSExpressionMammalianPromoterCMVAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
As3 (pBW411J117-arsR)
Plasmid#123142PurposeAn arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsRAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3
Plasmid#162918PurposeGateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)DepositorInsertfirefly luciferase/green fluorescent protein fusion with upstream IRES sequence
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SEPLuc-IRES-Antares
Plasmid#141085Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEPLuc-EMCV IRES-Antares
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-CuSeCat
Plasmid#42568DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Valin…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF’- AmTryoshka1;3-GS
Plasmid#100967PurposeYeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWP1_mFTCD
Plasmid#102317Purposeexpression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2DepositorAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-S10
Plasmid#127828PurposeProduction of recombinant human ribosomal protein S10DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
JM3 [JBEI-6891]
Plasmid#89788PurposeBroad host range vector pBBR1-MCS2 containing 'tesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli) under control of BAD promoterDepositorInserttesA (E. coli), fadB (E. coli), Mlut_11700 (M. luteus) and fadM (E. coli)
ExpressionBacterialAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC1-Rap2A-3xFLAG
Plasmid#87972PurposeExpression of Rap2A in mammalian cellsDepositorAvailable SinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-PRMT9 L182A/D183A/I184A/G185A
Plasmid#67608Purposebacterial expression of catalytically inactive GST-PRMT9 LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
TagsGSTExpressionBacterialMutationcatalytic mutant LDIG(182-185)AAAAPromotertacAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
KHBD00486
Plasmid#39591DepositorAvailable SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88-mut
Plasmid#87337PurposeTo express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP88 (NUP88 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM117: VEGFA array presgRNA
Plasmid#166869PurposeU6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting array presgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgShmt2_2
Plasmid#106315PurposeExpress Cas9 and sgRNA targeting mShmt2DepositorInsertsgRNA targeting mShmt2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta13 Aphrodite
Plasmid#13570DepositorInsertFLIPglu F16A Delta13 Aphrodite
TagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLPC myc-mRAP1 I312R
Plasmid#73137PurposeExpression of myc-tagged mRAP1 with single point mutatoin I312RDepositorInsertRap1 (Terf2ip Mouse)
TagsmycExpressionMammalianMutationisoleucine changed to an arginine at amino acid 3…Available SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-OAZ-GSY-SPM-Ctag
Plasmid#163613PurposeExpresses truncated human Ornithine Decarboxylase Antizyme fused to SPM in bacterial cells for calcium-induced covalent coupling to Ornithine Decarboxylase.DepositorInsertOAZ-GSY-SPM
TagsC-tag and His6ExpressionBacterialPromoterT7Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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OphA-delta C6
Plasmid#127679PurposeBacterial expression of His-tagged OphA-delta C6DepositorInsertOphA-delta C6
TagsHis8ExpressionBacterialMutationdelta C6Available SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
As1
Plasmid#123159PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MolBC
Plasmid#92210PurposeExpression of H. influenzae MolBC (ABC Transporter) in E. coliDepositorTags8x Histidine Tag and Enterokinase Cleavage Site b…ExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Neo-PTEN
Plasmid#16411DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
A102 ΔAmpH Δ2x+ve
Plasmid#129484PurposeExpression of Glo3 ΔAmpH lacking the COPI-binding motif in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
TagsHis and MBPExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5-FLAG-CaMKKbeta rat K193A
Plasmid#32450DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
TagsFLAGExpressionMammalianMutationK193A (S3V, G96R, and P108L also present)PromoterSV40Available SinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
KHBD00678
Plasmid#39725DepositorAvailable SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
ExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_delta ald1
Plasmid#126708Purposevector for knocking out ald1 in Komagataella phaffiiDepositorInsertHIS4
ExpressionYeastPromoterHisAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-S15
Plasmid#127822PurposeProduction of recombinant human ribosomal protein S15DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-NDUFB4
Plasmid#139840PurposeLentiviral expression of human NDUFB4DepositorInsertNDUFB4 (NDUFB4 Human)
UseLentiviralAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES mYFP
Plasmid#130712PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-F98-NLS IRES mYFP
TagsNLS and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG
Plasmid#132684PurposeAAV plasmid encoding mouse PM20D2 with C-terminal flag tagDepositorAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N1 cyclin B1 L45A (1324)
Plasmid#61851Purposemammalian expression of cyclin B1 L45 A mutant fused to VenusDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S15
Plasmid#127823PurposeProduction of recombinant human ribosomal protein S15DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.16
Plasmid#133536PurposesfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start siteDepositorInsertsfGFP
TagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 Sox4 WT 3'UTR oligo
Plasmid#26990DepositorInsertSox4 wt UTR oligo (SOX4 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationbp 449-509 of SOX4 3' UTRAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97 IRES EGFP
Plasmid#130707PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertNTOM20-SNAP-Tag-LAMA-G97 IRES EGFP
TagsNTOM20 and SNAP-TagExpressionMammalianAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLenti CRISPR sgSHMT2_2
Plasmid#106310PurposeExpress Cas9 and sgRNA targeting SHMT2DepositorInsertsgRNA targeting SHMT2
UseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shPIK3CD.v2 puro
Plasmid#58707PurposeLentiviral shRNA vector for knockdown of human PIK3CDDepositorAvailable SinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJH104
Plasmid#48262PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.DepositorInsertsTEF1p 5' fragment
URA3 5' fragment
TEF1p 3' fragment
UseRoutine cloning vectorMutationFrom SK1 background. -343 to -1 relative to TEF1 …PromoterTEF1pAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEM-rat Fcepsilon RI gamma
Plasmid#8375DepositorAvailable SinceJune 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSUPER.retro.puro-shMePCE
Plasmid#113536PurposeRetroviral vector designed to knock down MePCE.DepositorInsertshMePCE (MEPCE Human)
UseRetroviralAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
KHBD00549
Plasmid#39644DepositorAvailable SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -