159,157 results

1. pUC19-hAPOE-R-loop

   Plasmid

   #134901

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   APOE promoter (APOE Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRDB_347

   Plasmid

   #231114

   Purpose

   CBE activity-based selection cloning vector

   Depositor

   David Root, John Doench

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBlueKan+cysMPro

   Plasmid

   #187879

   Purpose

   Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

   Depositor

   Gaylen Uhlich

   Article

   Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CysM

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-hSNRPN-R-loop

   Plasmid

   #134902

   Purpose

   Generation and purification of R-loops for in vitro biochemistry

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   hSNRPN promoter (SNRPN Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FlucWT-HA-GFP11-N1

   Plasmid

   #91954

   Purpose

   Expresses FlucWT-HA-GFP11 in mammalian cells

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   FlucWT-HA-GFP11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS2 (hANGPTL4-V5)

   Plasmid

   #102446

   Purpose

   Expresses V5-tagged human ANGPTL4 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   ANGPTL4 (ANGPTL4 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LentiCRISPRv2-Opti

   Plasmid

   #163126

   Purpose

   LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-AUP1-FLAG

   Plasmid

   #185336

   Purpose

   Mammalian expression of AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Use

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

   Plasmid

   #196196

   Purpose

   homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
   

   Use

   Homology repair donor

   Tags

   V5-APEX2-mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHMR1A

    Plasmid

    #102957

    Purpose

    gene cloning of MR1A

    Depositor

    Lucia Mori, Gennaro De Libero

    Article

    Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

    Insert

    MR1A cDNA (MR1 Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Stop codon has been removed by HindIII site

    Promoter

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCherry-Integrin-Alpha2-N-18

    Plasmid

    #55063

    Depositor

    Michael Davidson

    Insert

    Integrin-Alpha2 (ITGA2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UKNP2.4.1

    Plasmid

    #98212

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-1-StUbi-6

    Plasmid

    #197710

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    StUbi promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB5319

    Plasmid

    #204829

    Purpose

    An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW1 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIRE YIC

    Plasmid

    #18784

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    H-ferritin iron response element
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-M-Flag-BAP1

    Plasmid

    #125840

    Purpose

    express wild type BAP1

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-mGFP

    Plasmid

    #14757

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Has Service

    Cloning Grade DNA

    Insert

    membrane bound form of EGFP
    

    Use

    Tags

    Palmitoylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-mGreenLantern

    Plasmid

    #164469

    Purpose

    Constitutive CAG-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CAG

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LeGO-hEµMAR-G2

    Plasmid

    #52633

    Purpose

    3rd generation lentiviral vector for enhanced eGFP expression/marking in primary B cells and B-cell lines

    Depositor

    Boris Fehse

    Article

    Mock et al Hum Gene Ther Methods. 2012 Dec;23(6):40

    Insert

    hEµMAR-SFFV; eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. deltaNp63alpha-FLAG

    Plasmid

    #26979

    Depositor

    David Sidransky

    Article

    Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

    Insert

    ΔNP63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    dominant negative isoform of p63; aa 1–587

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMXs-hs-EGR1

    Plasmid

    #52724

    Purpose

    retroviral expression of human EGR1

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET His6 NusA TEV LIC cloning vector (2N-T)

    Plasmid

    #29709

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-NusA-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. dTAG-IRF8

    Plasmid

    #190620

    Purpose

    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12_G36V

    Expression

    Mammalian

    Mutation

    synonymous mutation for sgRNA resistance for sgIR…

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCSFLPe

    Plasmid

    #31130

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONRPEX18Tp-SceI-pheS

    Plasmid

    #111608

    Purpose

    Gateway compatible allelic exchange vector for Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Type

    Empty backbone

    Use

    Allelic exchange in burkholderia sp.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJQ200SK

    Plasmid

    #78497

    Purpose

    Suicide vector for gene replacement

    Depositor

    Michael Hynes

    Article

    Quandt et al Gene. 1993 May 15;127(1):15-21.

    Type

    Empty backbone

    Use

    Suicide plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-Flag Rac2

    Plasmid

    #12192

    Depositor

    Gary Johnson

    Article

    Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

    Insert

    Rac2 (RAC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221_SLC2A2

    Plasmid

    #132126

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA1 Flag ATXN1[30Q]

    Plasmid

    #33236

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 29 amino acids (27 Q…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

32. EF1a mCherry

    Plasmid

    #129431

    Purpose

    expression of mCherry from EF1a promoter

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMRE132

    Plasmid

    #118486

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MS2-Snapf

    Plasmid

    #65860

    Purpose

    Expresses RNA-binding protein MS2 with fast SNAP fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    Fast SNAP
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

35. dSpCas9-VPR

    Plasmid

    #205247

    Purpose

    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Use

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherry Vinculin

    Plasmid

    #50527

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-ABCB1_STOP

    Plasmid

    #221424

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCB1 (ABCB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge