We narrowed to 40,651 results for: TRO

1. pALPS_3'LTR GFP@gag start SFFV rev

   Plasmid

   #101339

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized rev

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUPD2 SV40-AcrIIA4 (GB3333)

   Plasmid

   #160605

   Purpose

   SV40-AcrIIA4 codon optimized for N.benthamiana

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LC3-mEYFP

   Plasmid

   #182865

   Purpose

   Autophagy Marker

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

   Insert

   rat LC3 (Anxa3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

   Plasmid

   #133334

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLJM1-zeo-2xStrep-BFLF1

   Plasmid

   #162661

   Purpose

   Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   BFLF1
   

   Use

   Lentiviral

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP Snail 8SA

   Plasmid

   #16229

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pL59-Nat

   Plasmid

   #140465

   Purpose

   CRISPR/Cas9 engineering with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGAD-GH-MEFV

   Plasmid

   #92106

   Purpose

   Yeast two hybrid

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   MEFV (MEFV Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS NL4-3 envFS eGFP Δgag/pol

   Plasmid

   #101343

   Purpose

   Deletion from the start of gag until 229 bases before the cPPT

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta GagPol

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSR9.1347

    Plasmid

    #39540

    Depositor

    Katharina Strub

    Article

    Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

    Insert

    SRP9 (SRP9 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. RV-Cag-Dio-mEYFP

    Plasmid

    #87666

    Purpose

    retroviral vector encoding cre dependent expression of membrane EYFP

    Depositor

    Lynette Lim, Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Mem-EYFP
    

    Use

    Retroviral

    Tags

    palmitylation

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BC-A1-004

    Plasmid

    #78691

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BC-A1-003

    Plasmid

    #78690

    Purpose

    A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FLAG-mTUT2

    Plasmid

    #60039

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT2

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT2 (Papd4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

16. miniSOG-Display-5

    Plasmid

    #54822

    Purpose

    Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    miniSOG-Display

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pPU-Empty-miR30-L1221

    Plasmid

    #132940

    Purpose

    All-in-one control rescue-control shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-HA-MKRN1 H307E

    Plasmid

    #78749

    Purpose

    To overexpress MKRN1 H307E in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H307E

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSuper mouse DGCR8-3

    Plasmid

    #14774

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    DGCR8 shRNA (Dgcr8 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1(+) EPHB4 Sense

    Plasmid

    #60638

    Purpose

    Expresses the human EPHB4 exon 11/12 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ptreCas9-mKate2ps-T1gRNA

    Plasmid

    #62715

    Purpose

    Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGFPmAID_htz-1

    Plasmid

    #174214

    Purpose

    Donor plasmid for tagging htz-1 with GFP::mAID

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Insert

    GFP::mAID::htz-1 tagging donor
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. VV221: sdChR(C138S)-TS-eGFP-ER in fck

    Plasmid

    #58513

    Purpose

    Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-eGFP-ER
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRibo-Tt

    Plasmid

    #154124

    Purpose

    Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Inserts

    engineered bacterial rRNAs operon under control of pL promotor
    bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and pL

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKC123

    Plasmid

    #134240

    Purpose

    expresses gRNA targeting CENP A 3'

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP A 3' CRISPR construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKT-SCFP3A-HIS

    Plasmid

    #64686

    Purpose

    pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    SCFP3A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Nucleotide sequence has been yeast-codon optimize…

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BC-A1-013

    Plasmid

    #78701

    Purpose

    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

    Depositor

    Brian Chow

    Article

    Magaraci et al ACS Synth Biol. 2016 Apr 25.

    Insert

    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRFP shRNA-resistant TrkB WT

    Plasmid

    #72714

    Purpose

    Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

    Depositor

    Freddy Jeanneteau

    Article

    Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

    Insert

    TrkB
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

    Promoter

    CMV

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pHR'-ROMK1-CITE-GFP

    Plasmid

    #21322

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

    Insert

    ROMK1 (Kcnj1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

30. pET15b+PCNA1 WT

    Plasmid

    #66139

    Purpose

    Expresses the wild type of Sulfolobus solfataricus PCNA1

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDNA-FRT-FAK-kinase0

    Plasmid

    #59122

    Purpose

    FAK fret control sensor without FERM domain with no linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENTR-R4R3-3xmRuby-N

    Plasmid

    #113754

    Purpose

    Gateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmRuby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TC393

    Plasmid

    #122625

    Purpose

    Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CAG-EGFP-Otx2shRNA2

    Plasmid

    #73982

    Purpose

    Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    EGFP-Otx2-shRNA2
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AEB-DNT3

    Plasmid

    #63852

    Purpose

    This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    DNT3 DNT riboswitch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    AEB3

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKT-mCitrineV163A-HIS

    Plasmid

    #64685

    Purpose

    pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

    Depositor

    Erin O'Shea

    Article

    Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

    Insert

    mCitrine V163A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Valine 163 changed to alanine

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTL472

    Plasmid

    #69882

    Purpose

    dCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassette

    Depositor

    Robert Kittel, Tobias Langenhan

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Transformation vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CAG-mCherry-CrxshRNA

    Plasmid

    #73987

    Purpose

    Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Crx gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pILGFP9E3

    Plasmid

    #185842

    Purpose

    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEX-6p-1 Sesn1b

    Plasmid

    #61874

    Purpose

    bacterial expression of a GST-Sesn1b (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn1 (Sesn1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pKM149

    Plasmid

    #134178

    Purpose

    Bacterial expression of his-CENP-C 510-end

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 510-end (CENPC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 510-end

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. GFP Snail 4SA

    Plasmid

    #16227

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    4SA: S108A, S112A, S116A, S120A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

43. pJP102

    Plasmid

    #176480

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > NatR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJP103

    Plasmid

    #176481

    Purpose

    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

    Depositor

    Duojia Pan

    Article

    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

    Inserts

    actin > HygR
    EF1 > mScarlet
    

    Use

    Capsaspora owczarzaki expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Tol2 8xSTAR-mScarletI. PGK-puro

    Plasmid

    #136266

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104844

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    AVPR2-eLOV-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. pUC19-Top-Toehold-mCherry

    Plasmid

    #87318

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. CAG-EGFP-Otx2shRNA1

    Plasmid

    #73981

    Purpose

    Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    shRNA that targets the mouse Otx2 gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. CRISPRmTmG2

    Plasmid

    #69992

    Purpose

    The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA that targets near LoxP sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pALPS_3'LTR GFP@gag start SFFV tat

    Plasmid

    #101338

    Purpose

    LTR drives GFP and internal SFFV promoter drives codon optimized tat

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GFP, tat
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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