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172,031 results

Showing: 11151 - 11200 of 172031 results

  1. TAAR5-Tango

    Plasmid
    #66510
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TAAR5 (TAAR5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. TAAR6-Tango

    Plasmid
    #66511
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TAAR6 (TAAR6 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pTP943

    Plasmid
    #104157
    Purpose
    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG1 Fab specific nanobody TP886
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Available Since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104157-plasmid-map-sequence-id-200077

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  12. pENTR-R2L3-IRES-Zeo-pA

    Plasmid
    #139530
    Purpose
    Provides IRES-Zeo-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Zeocin resistant gene
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139530-plasmid-map-sequence-id-274699

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  13. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184957-plasmid-map-sequence-id-376681

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  14. pEf1a-goldDn29-dCas9-P2A-GFP

    Plasmid
    #247157
    Purpose
    Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Article
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Insert
    goldDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N
    Promoter
    Ef1a
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247157-plasmid-map-sequence-id-491683

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  15. pRK5-myc-RhoA-Q63L

    Plasmid
    #12964
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q63L Constitutively active
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12964-plasmid-map-sequence-id-139200

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  16. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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  18. pLENTI_hACE2_PURO

    Plasmid
    #155295
    Purpose
    Lentiviral vector to generate hACE2 stable expressing cell line
    Depositor
    Raffaele De Francesco
    Insert
    hACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    C9
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155295-plasmid-map-sequence-id-307855

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  24. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Article
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  29. CHIKV eGFP-Zeo replicon

    Plasmid
    #249289
    Purpose
    SP6-driven CHIKV_LR2006 OPY1 replicon with eGFP-Zeocin reporter selection cassette
    Depositor
    Amy Kistler
    Insert
    CHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes
    Expression
    Bacterial
    Mutation
    structural polyprotein open reading frame deleted…
    Promoter
    SP6
    Available Since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249289-plasmid-map-sequence-id-497969

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  30. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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  38. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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  40. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis and GST
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11520-plasmid-map-sequence-id-324017

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  43. Tet-O-FUW-FOXA2-PURO

    Plasmid
    #251737
    Purpose
    Overexpression construct expressing FOXA2-PURO and ORF-BC
    Depositor
    Andrea Califano
    Insert
    FOXA2 (FOXA2 Human)
    Use
    Lentiviral
    Available Since
    March 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    251737-plasmid-map-sequence-id-501493

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  45. TtEnd5A_pEHISTEV

    Plasmid
    #179732
    Purpose
    TtEnd5A_pEHISTEV expression vector encodes first Thermogutta terrifontis Endoglucanase (19-531 amino acid residues) of Glycoside Hydrolase Family 5.
    Depositor
    James Naismith
    Article
    Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027
    Insert
    TtEnd5A
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    19 Amino acids from N-terminal encoding Signal pe…
    Promoter
    T7
    Available Since
    Oct. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    179732-plasmid-map-sequence-id-357162

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  46. NRF2-SmBiT_MAFG-LgBiT BiBiT Vector

    Plasmid
    #238773
    Purpose
    Express NRF2-SmBiT_MAFG-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRF2 (NFE2L2 Human)
    MAFG (MAFG Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238773-plasmid-map-sequence-id-479907

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  47. HaloTag-JAK3 Vector

    Plasmid
    #238701
    Purpose
    Express HaloTag-JAK3 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    JAK3 (JAK3 Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238701-plasmid-map-sequence-id-479800

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  49. NanoBRET Assay Vector, NanoLuc-MLL1

    Plasmid
    #237135
    Purpose
    Express NanoLuc(R)-MLL1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MLL1 (KMT2A Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237135-plasmid-map-sequence-id-476791

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Showing: 11151 - 11200 of 172031 results