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177,170 results

Showing: 11201 - 11250 of 177170 results

  2. pHCMV_COCVmut_G

    Plasmid
    #207262
    Purpose
    Mammalian expression of a mutant Cocal virus G protein
    Depositor
    Feng Zhang
    Publication
    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COCVmut_G
    Expression
    Mammalian
    Mutation
    K47A/Y209A/R354A; mammalian codon optimization
    Promoter
    CMV promoter
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207262-plasmid-map-sequence-id-407611

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  4. FSW_jGCaMP8f

    Plasmid
    #197034
    Purpose
    Express GCaMP8f from hSyn1 promoter
    Depositor
    Ronald Hart
    Insert
    GCaMP8f
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    197034-plasmid-map-sequence-id-392710

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  9. pCMV-eNme2-T.1-ABE8e

    Plasmid
    #185668
    Purpose
    Expresses eNme2-T.1-ABE8e in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA(8e)-neNme2-T.2
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185668-plasmid-map-sequence-id-373131

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  11. CMV-Nme2ABE8e

    Plasmid
    #189928
    Purpose
    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.
    Depositor
    David Liu
    Publication
    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nme2ABE8e
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Nme2Cas9 D16A
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189928-plasmid-map-sequence-id-371173

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  13. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    182287-plasmid-map-sequence-id-363047

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  15. pGL4.10/RSV_SF3B3-Xon_Luciferase

    Plasmid
    #174659
    Purpose
    Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.
    Depositor
    Beverly L. Davidson
    Publication
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Xon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174659-plasmid-map-sequence-id-351621

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  19. pGEX-GST-His6-2_HBD

    Plasmid
    #171870
    Purpose
    Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells
    Depositor
    Kaiwei Liang
    Publication
    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe
    Insert
    Tandem repeats Hybrid binding domain of Rnase H1
    Tags
    GST- and His10-tag
    Expression
    Bacterial
    Mutation
    WT
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171870-plasmid-map-sequence-id-338468

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  22. pDONRp5-p2 GFP-NShroom3-iLID

    Plasmid
    #170976
    Purpose
    N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Publication
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NShroom3 (Shroom3 Mouse)
    Use
    Gateway Cloning
    Tags
    eGFP and iLID
    Mutation
    From isoform 2, deleted amino acids 1389 - 1805,…
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170976-plasmid-map-sequence-id-336790

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  28. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid
    #161926
    Purpose
    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    rTetR(SE-G72P)
    Use
    Lentiviral and Synthetic Biology
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    161926-plasmid-map-sequence-id-324346

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  29. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

    Plasmid
    #164514
    Purpose
    Green glucose sensor with mRuby2 fusion, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3 (Plasmid #244084) instead of this plasmid.
    Depositor
    Loren Looger
    Publication
    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi
    Insert
    (Cyto)iGlucoSnFR-mRuby2
    Use
    AAV
    Mutation
    Synthetic Construct
    Promoter
    GFAP
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    164514-plasmid-map-sequence-id-324004

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  32. GFP-OMP25

    Plasmid
    #141150
    Purpose
    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells
    Depositor
    Gia Voeltz
    Publication
    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OMP25 (Synj2bp Rat)
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    OMP25 c terminus from addgene plasmid #69598
    Promoter
    CMV
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    141150-plasmid-map-sequence-id-309903

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  41. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    138566-plasmid-map-sequence-id-274798

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  42. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Publication
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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  47. pCMV-Sport6-CD9-pHluorin

    Plasmid
    #130905
    Purpose
    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD9-pHluorin (CD9 Aequorea victoria, Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    phluorin inserted between Asn-50 and Asn-51 in ex…
    Promoter
    CMV
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    130905-plasmid-map-sequence-id-272542

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  49. pAAVS1-TRE-MA4-GFP

    Plasmid
    #114380
    Purpose
    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.
    Depositor
    Yuet Wai Kan
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MLL-AF4 fusion gene (AFF1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    TRE
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    114380-plasmid-map-sequence-id-220669

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Showing: 11201 - 11250 of 177170 results