We narrowed to 26,677 results for: Cat

1. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTargeting_Mettl3C_FKBP-V

   Plasmid

   #165421

   Purpose

   Template for knockin FBKP-V into Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
   

   Use

   Mouse Targeting

   Tags

   FKBP-V

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SNX29-PX (269-391)

   Plasmid

   #119108

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX29-PX (269-391)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. shTBX5 # 2

   Plasmid

   #42550

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTBX5 (TBX5 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. shTBX5 # 1

   Plasmid

   #42549

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   shTBX5 (TBX5 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. NLS-dCas9-trCIB1

   Plasmid

   #64119

   Purpose

   Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   dCas9-trCIB1 fusion
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   dCas9 (D10A H840A, catalytically inactive), trCIB…

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-6P-1 SARS-CoV 3CLpro

   Plasmid

   #61692

   Purpose

   inducible expression of SARS 3CLpro protease

   Depositor

   Michael Johnson

   Article

   Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

   Insert

   SARS 3CLpro protease
   

   Use

   Tags

   GST and HIS

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pPBO.ACT006

   Plasmid

   #182716

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   GFP in cloning site of crRNA for easier screening…

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6598-HisSUMO1GG

    Plasmid

    #13376

    Depositor

    Anne Dejean

    Article

    Seeler et al Mol Cell Biol. 2001 May . 21(10):3314-24

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    T7 in vitro translation

    Tags

    His

    Expression

    Mammalian

    Mutation

    mature form ending with GG

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB CAG Otx2-IRES-EGFP

    Plasmid

    #153947

    Purpose

    piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

    Depositor

    Luca Tiberi

    Article

    Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

    Insert

    Otx2 (Otx2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ATG9A-EGFP

    Plasmid

    #210367

    Purpose

    Expresses ATG9A fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pORANGE Bsn-GFP KI

    Plasmid

    #139665

    Purpose

    Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IDE-2Strep

    Plasmid

    #210355

    Purpose

    Expresses IDE fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    IDE (IDE Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-gp78c/2767

    Plasmid

    #11429

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    gp78c (AMFR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMuLE ENTR SV40 PuroR L3-L2

    Plasmid

    #62164

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Puromycin
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199366

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. STARR-seq luciferase validation vector_mP_empty

    Plasmid

    #99298

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-OT (wt TCF-4 sites)

    Plasmid

    #16558

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    TCF-4 binding sites (TCF4 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    3 copies of wildtype Tcf-4 binding sites

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PresentER-NLVPMVATV (GFP)

    Plasmid

    #102947

    Purpose

    PresentER minigene encoding HLA-A*02:01 ligand NLVPMVATV (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Cancer Immunol Res. 2020 May;8(5):672-68

    Insert

    NLVPMVATV
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTrc99A-Rat FABP1

    Plasmid

    #13577

    Depositor

    Martin Scanlon

    Article

    Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

    Insert

    Rat liver fatty acid binding protein (Fabp1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

23. "AND-gate" receiver plasmid

    Plasmid

    #127676

    Purpose

    Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxR, GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 for LuxR, pLux for GFP

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCS2+ Zf GFP-vclb

    Plasmid

    #185411

    Purpose

    GFP tagged form of zebrafish vinculin b. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin b (vclb Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pINDUCER21-HOXA9

    Plasmid

    #51302

    Purpose

    Expression of HOXA9 after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    homeobox A9 (HOXA9 Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    n/a

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMJ923

    Plasmid

    #78313

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Use

    Tags

    HA-2xNLS-mCherry-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 2xHP1bCD_W42A-IFP2.0C

    Plasmid

    #215751

    Purpose

    Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    2xHP1bCD_W42A-IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGTM_COV2_NSP5_004_SUMO

    Plasmid

    #190062

    Purpose

    Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    3CLpro (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLX-B3omega1 (GB1694)

    Plasmid

    #160643

    Purpose

    pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

    Depositor

    Fabio Pasin, Juan Antonio García

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCS2+ Zf GFP-vcla

    Plasmid

    #185410

    Purpose

    GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    vinculin a (vcla Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPBC-G6s

    Plasmid

    #62812

    Purpose

    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP6s-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Axin2-luciferase

    Plasmid

    #21275

    Depositor

    Frank Costantini

    Article

    Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

    Insert

    Axin2 promoter (Axin2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

34. p-EGFP-C1-Flag-mFmr1(A)

    Plasmid

    #87913

    Purpose

    Expresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.

    Depositor

    Stephanie Ceman

    Article

    Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    S499A

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEX-KG-Itch/2793

    Plasmid

    #11434

    Depositor

    Allan Weissman

    Article

    Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

    Insert

    Itch (Itch Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    n/a

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

    Plasmid

    #100279

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Mutated catalytic domain (1025aa-End) (please see…

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Axin2-d2EGFP

    Plasmid

    #21276

    Depositor

    Frank Costantini

    Article

    Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

    Insert

    Axin2 promoter (Axin2 Mouse)
    

    Use

    Tags

    d2EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 5HT6-YC3.60

    Plasmid

    #48339

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
    

    Use

    Tags

    YC3.60

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p-EGFP-C1-Flag-mFmr1(D)

    Plasmid

    #87914

    Purpose

    Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

    Depositor

    Stephanie Ceman

    Article

    Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

    Insert

    Fmr1 (Fmr1 Mouse)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    S499D

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. EGFP-Ahi1

    Plasmid

    #30494

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMGIB-3xFLAG-pontin

    Plasmid

    #53608

    Purpose

    Retroviral expression of human pontin

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    pontin (RUVBL1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    pontin CDS mutated to confer resistance to shRNA.…

    Promoter

    LTR

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

44. pORANGE GFP-Bsn KI

    Plasmid

    #139664

    Purpose

    Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA4-Beclin1 243-450

    Plasmid

    #24392

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 243-450.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

46. pcDNA4-Beclin1 151-241

    Plasmid

    #24390

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains amino acids 151-241.

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pME V5-mTagBFP2 (JDW 830)

    Plasmid

    #224484

    Purpose

    Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mTagBFP2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #202610

    Purpose

    Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    tdTomato
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. 3xFLAG-dCas9/pMXs-neo

    Plasmid

    #51260

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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