19,773 results
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Plasmid#100848PurposeAAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1 and AAV9InsertjRCaMP1a
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
TagsHis6ExpressionBacterialPromoteraraBpAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1337
Plasmid#119270Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme WT
Plasmid#18110DepositorInsertT4 Lysozyme (e Bacteriophage T4)
ExpressionBacterialAvailable SinceAug. 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19 tandem CAR
Plasmid#113019Purposefor lentivirus production of CD19 tandem CARDepositorInsertCD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Mouse, Synthetic, Human)
UseLentiviralTagsGFPExpressionMammalianAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VSVM
Plasmid#64086PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain matrix geneDepositorInsertVSV matrix protein
ExpressionMammalianPromoterCAGAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HIV RT-6xHis-RBS-prot
Plasmid#159149Purposeexpresses His-tagged HIV reverse transcriptase and untagged HIV proteaseDepositorInsertsHuman immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease
TagsHisExpressionBacterialMutationHIV-1 group M/subtype B – BH10 strainAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
TagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/opto-b2AR-EYFP
Plasmid#20948PurposeMammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signalingDepositorInsertopto-b2AR
ExpressionMammalianAvailable SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
ADRB1-Tango
Plasmid#66219PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP 3xFLAG-VMP1
Plasmid#117416PurposeExpresses VMP1 tagged with 3xFLAG in mammalian cellsDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Merlin
Plasmid#84293PurposeExpression of wt MerlinDepositorAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0 FLAG TRPV4
Plasmid#45751DepositorAvailable SinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Synthetic, Human)
TagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NORAD
Plasmid#120383PurposeExpression vector for lncRNA NORADDepositorAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0042-PguCas13b crRNA backbone
Plasmid#103853PurposeFor cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caacDepositorInsertU6-PguCas13b DR-BbsI-BbsI-polyT
UseCRISPRExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.tMCK.PI.eGFP.WPRE.bGH
Plasmid#105556PurposeAAV expression of EGFP from tMCK promoterDepositorHas ServiceAAV9InsertEGFP
UseAAVExpressionMammalianPromotertMCKAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3
Plasmid#110099PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage siteDepositorTypeEmpty backboneTagsHisExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-ldhGFP
Plasmid#27170DepositorInsertlactate dehydrogenase promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-HA-humanCMYC
Plasmid#74164PurposeMammalian expression of human cMycDepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 MT
Plasmid#78261PurposepsiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated let-7 target site
UseLuciferase; Microrna activityExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CTCC to GA…Available SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-mpeg1-dendra2
Plasmid#51462Purposeexpresses photoconvertible dendra in macrophagesDepositorInsertmpeg-dendra
ExpressionBacterialPromotermpeg1Available SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#125692PurposeGlobal labeling of inhibitory synapses (green fluorescence)DepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterEF1AAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-WT
Plasmid#91978PurposeExpresses FLAG-HA-AGO2 (WT)DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS1089: mini-AAV.sgRNA.Nme2Cas9
Plasmid#159536PurposeDelivery of Nme2Cas9 and its sgRNA in a single AAV vector with overall packaging size of 4.4 KbDepositorInsertNme2Cas9 with single guide RNA cassette
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only