177,170 results
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Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralPromoterPGKAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-RAB10
Plasmid#130883PurposeExpresses human RAB10 with N-terminal GFP tagDepositorAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO APEX2-GFP
Plasmid#129640PurposeExpress APEX2-GFP fusion in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPEX2-GFP
ExpressionMammalianAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorPromoterP23Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRX-INU-FLAG-FAM134B
Plasmid#128260PurposeExpresses FAM134B in mammalian cellsDepositorAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LV-Sox10MCS5-GFP
Plasmid#115783PurposeThis plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.DepositorInsertmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
UseLentiviralTagscfos basal promoter conjugated MCS5 promoter fuse…PromoterSOX10 Multiple Species Conserved enhancer element…Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-mNeonGreen
Plasmid#99131PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mNeonGreenDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti F9-TetR-EGFP-IRES-PuroR
Plasmid#117049Purposelentiviral vector for expression of TetR-EGFP, for visualization of TetO repeatsDepositorInsertF9-TetR-EGFP
UseLentiviralTagsEGFPExpressionMammalianPromoterF9 promoterAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K27R
Plasmid#121155PurposeMammalian expression of HA tagged ubiquitin K27RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112669PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…ExpressionMammalianAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyAvailable sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-dLight1.1
Plasmid#111067PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoterDepositorHas serviceAAV1 and AAV5InsertdLight1.1
UseAAVTagsFlag tagAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti NL
Plasmid#113450PurposeLentiviral NanoLuc control expression vectorDepositorInsertNanoLuc
UseLentiviral and LuciferaseTagscmycExpressionMammalianPromoterhUbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-puro-shNT
Plasmid#109012Purposeshort-hairpin knockdown of targeted geneDepositorInsertNon-targeting shRNA
UseLentiviralExpressionMammalianPromoterhU6 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUS-Citrine-PixE
Plasmid#111505PurposeFor PixELL systemDepositorAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsFLAGExpressionMammalianAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-P(Cry1)-forward-intron336-Venus-NLS-D2
Plasmid#110054PurposeCry1 transcription fluorescence reporterDepositorAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsHAExpressionMammalianAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE
Plasmid#104491PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX551-miniCMV-SpCas9
Plasmid#107031PurposeAAV plasmid expressing Cas9 under control of miniCMV promoterDepositorInsertSpCas9
UseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN3-Nipbl
Plasmid#107716PurposeExpression plasmid for 3xFLAG-tagged NIPBLDepositorAvailable sinceMarch 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-hSynI-rtTAV16
Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGART-GFP
Plasmid#99106PurposeExpresses human GART-GFP fusion in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGART (GART Human)
TagsGFPExpressionMammalianMutationA65T, L68V, and A72S mutations in GFP gene; kanR/…PromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT miniAID-EGFP
Plasmid#101713PurposeFor amplification of a miniAID-EGFP for C-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminiAID-EGFP
TagsminiAID-EGFPExpressionMammalianAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHel2
Plasmid#102960PurposeE.coli/H.Pylori shuttle vectorDepositorTypeEmpty backboneUsePlasmid shuttle vector system for genetic complem…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTurquoise2-LaminB
Plasmid#98830PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLaminB
TagsmTurquoise2ExpressionMammalianMutation*(see below)PromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A-polyA
Plasmid#101051Purposein vitro transcription (T7 promoter)DepositorAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPAICS-EGFP
Plasmid#99108PurposeExpresses human PAICS-EGFP fusion in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPAICS (PAICS Human)
TagsEGFP (enhanced green fluorescent protein)ExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1/Puro-CAG-ASAP2s
Plasmid#101274PurposeGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertASAP2s
ExpressionMammalianPromoterCAGAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CBh-DIO-EGFP
Plasmid#87168PurposeExpression of eGFP in the presence of CreDepositorInsertEGFP
UseAAVTagsNoneAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BCL2L1_pLX307
Plasmid#98323PurposeLentiviral expression of BCL2L1DepositorAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KillerOrange /pQE-30
Plasmid#74748PurposeKillerOrange gene for bacterial expressionDepositorInsertKillerOrange
TagsHisTagExpressionBacterialMutationKillerRed with substitutions G3C Y66W D113S N145S…PromoterT5Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAsCpf1(TYCV)(BB) (pY211)
Plasmid#89352PurposeExpresses humanized AsCpf1 TYCV PAM variant and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-FLEX-eNpHR-EYFP
Plasmid#89876PurposeTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoterDepositorInserteNpHR=EYFP
UseAAVTagsEYFPExpressionMammalianPromoterTetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_NOTCH2_WT
Plasmid#82292PurposeGateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2-GFP-homer1c
Plasmid#89442PurposeLocalizes CRY2 to excitatory synapsesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2-GFP-homer1c
ExpressionMammalianMutationContains Full length CRY2PromoterCAGAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ141-ZmUbi-RZ-As
Plasmid#86196PurposeAsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertAsCpf1 gRNA cloning site for ribozyme cleavage
UseCRISPRTagsHammerhead ribozyme and HDV ribozymeExpressionPlantPromoterMaize ubiquitin 1Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET32bplus_trx_dAChE4
Plasmid#83917PurposeEncodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coliDepositorInsertDesigned hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
TagsHis, S-tag, and TRXExpressionBacterialMutationV13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…PromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFG4
Plasmid#87251Purposegene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-HphDepositorInsertHyg
TagsparS segmentsExpressionYeastPromoterTEFAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNAHygro(+)LAMP2B
Plasmid#86029PurposeExpresses LAMP2B in Mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLysosome-associated membrane protein 2b (LAMP2 Human)
ExpressionMammalianPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by