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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Anti-Cav3.2 Ca2+ channel [N55/10R]

Plasmid

#177562

Purpose

Mammalian Expression Plasmid of anti-Cav3.2 Ca2+ channel (Human). Derived from hybridoma N55/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav3.2 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNA1H Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-NE

Plasmid

#215486

Purpose

Lentiviral expression of human alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

NE tag

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Human, Synthetic)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pPGK-empty

Plasmid

#35094

Depositor

David Segal

Article

Bhakta et al Methods Mol Biol. 2010;649:3-30.

Insert

deletion of zinc fingers and nuclease

Tags

HA

Expression

Mammalian

Mutation

use as a transfection balance for pPGK-ZFN plasmi…

Promoter

PGK

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe-GFP-IKBalpha-mut (super repressor)

Plasmid

#15264

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKB alpha (NFKBIA Human)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pQ2aB

Plasmid

#124887

Purpose

Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP

Depositor

Nevil Singh

Type

Empty backbone

Use

Retroviral

Tags

Blue Flourescent Protein, Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQFT-mNG

Plasmid

#203675

Purpose

pQFT with mNeonGreen under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

mNeonGreen

Expression

Bacterial

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-EGFP-3xHA (JDW 1322)

Plasmid

#224494

Purpose

Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Liefact-EGFP-3xHA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Promoter

dHsp70

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29.4R]

Plasmid

#114518

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCX-TRI-2A

Plasmid

#74674

Purpose

mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Inserts

HO1 (HMOX1 Human)
CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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KRAB-dCas9

Plasmid

#112195

Purpose

KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

KRAB (ZNF10 Human)

Use

CRISPR

Tags

3XFLag-NLS-KRAB-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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Active Wnt1-V5

Plasmid

#43807

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

Wnt1 (WNT1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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JC Mad-1

Plasmid

#25626

Depositor

Peter Howley

Article

Howley et al J Virol. 1980 Dec . 36(3):878-82.

Insert

JC Virus Mad-1

Expression

Bacterial

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pPOTv7-blast-CLIPtag

Plasmid

#179787

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

CLIP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pYY10-2µ-TP901

Plasmid

#235771

Purpose

2µ plasmid for constitutive expression of TP901 under RNR2 promoter.

Depositor

Chang Liu

Article

Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

Insert

TP901 integrase

Expression

Yeast

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pYY13-URA3-TP901LP

Plasmid

#235772

Purpose

Plasmids for generation of donor DNA for construction of a TP901-compatible landing pad p1 with URA3 selection marker.

Depositor

Chang Liu

Article

Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

Insert

URA3

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pYY62-P1int-TP901-GFPdropout

Plasmid

#235773

Purpose

Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.

Depositor

Chang Liu

Article

Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

Type

Empty backbone

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Human, Synthetic)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pC3.1-caggs-Crimson-CAAX

Plasmid

#190041

Purpose

Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Depositor

Michael Lin

Article

Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

Insert

Crimson

Tags

CAAX

Expression

Mammalian

Promoter

caggs

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (2B-T)

Plasmid

#29666

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pLJM5-WT PHGDH

Plasmid

#83901

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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CRISPR-StAR1 (Retro)

Plasmid

#222687

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

Plasmid

#216668

Purpose

Expresses membrane-bound mCherry in mammalian cells

Depositor

Leonardo Morsut

Insert

mCherry

Tags

PDGFR Transmembrane Domain

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-puro-mIR-31

Plasmid

#26088

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2010

Availability

Academic Institutions and Nonprofits only
pSLQ10878

Plasmid

#183964

Purpose

CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D156R, E292R, D235R, D350R

Promoter

CAG

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABA(A)R, Beta3 [N87/25R]

Plasmid

#177573

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Beta3 (Mouse). Derived from hybridoma N87/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Beta3 (Mus musculus) recombinant mouse monoclonal antibody (Gabrb3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM16F-M522K-peGFP-N1

Plasmid

#228579

Purpose

Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

M522K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

Plasmid

#170177

Purpose

Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2s-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-PB-PGK-tetR-KRAB-2A-Puro

Plasmid

#129536

Purpose

PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

PB-PGK-tetR-KRAB-2A-Puro

Use

Unspecified

Promoter

hPGK promoter

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_526

Plasmid

#184859

Purpose

Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
phOct4-EGFP

Plasmid

#38776

Depositor

Wei Cui

Article

Gerrard et al Stem Cells. 2005;23(1):124-33.

Insert

Oct-4 promoter (POU5F1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

human OCT4

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_mGL_PP7

Plasmid

#232440

Purpose

expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

mGreenLantern

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO/Strep-HA-ZAKalpha_K45A

Plasmid

#141194

Purpose

Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

Changed Lysine 45 to Alanine

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Con/Fon oG

Plasmid

#131778

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

rabies glycoprotein - oG

Use

AAV; Intrsect

Tags

none

Expression

Mammalian

Promoter

Ef1a

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141B2.0

Plasmid

#99897

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR human Drosha1

Plasmid

#14766

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB1-A

Plasmid

#190114

Purpose

Level 1 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM040 [Multiome Perturb-seq sgRNA expression vector]

Plasmid

#224898

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

Depositor

Thomas Norman

Article

Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE687

Plasmid

#167299

Purpose

Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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