165,307 results

1. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-GFP-dynein-2(D1091–Q4307)

   Plasmid

   #64064

   Purpose

   Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

   Depositor

   Andrew Carter

   Article

   Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

   Insert

   Cytoplasmic dynein-2 (DYNC2H1 Human)
   

   Tags

   GFP, His tag, and ZZ tag

   Expression

   Insect

   Mutation

   Deleted amino acids 1-1090, extra C-terminal vali…

   Promoter

   Polyhedrin

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmiRFP713-N1

   Plasmid

   #136559

   Purpose

   A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP713
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR1A no ccDB (w48-1)

   Plasmid

   #17398

   Purpose

   Gateway entry 1A vector, no ccDB gene

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_SMURF2_WT

   Plasmid

   #82259

   Purpose

   Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMURF2 (SMURF2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-GFP-MT2A

   Plasmid

   #11613

   Depositor

   Steven Johnson

   Insert

   metallothionein 2A (MT2A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-LMP1-IRES-GFP

   Plasmid

   #220350

   Purpose

   Expresses EBV latent gene LMP1 in mammalian cells

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS3Clux

   Plasmid

   #55172

   Purpose

   lux-reporter vector, integration at sacA, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Ubiquitin

    Plasmid

    #187910

    Purpose

    Mammalian cell expression of EGFP-tagged Ubiquitin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    UBB (UBB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

    Plasmid

    #207861

    Purpose

    AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB
    

    Use

    AAV

    Mutation

    See Manuscript

    Promoter

    Cbh

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170809

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

    Plasmid

    #170810

    Purpose

    Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET11a-Z-NspGFP

    Plasmid

    #40729

    Depositor

    Brian McNaughton

    Article

    Blakeley et al Mol Biosyst. 2012 Aug;8(8):2036-40. Epub

    Insert

    N super positive GFP - Leucine Zipper
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti CMV/TO Neo empty (w215-1)

    Plasmid

    #17485

    Purpose

    3rd gen lentiviral empty control vector, CMV/TO promoter, Neo

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV-YFP

    Plasmid

    #26000

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    H2B-YFP (H2BC21 A. victoria, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pECE-M2-BAIAP2 wt

    Plasmid

    #31656

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 (BAIAP2 Human)
    

    Tags

    M2

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTK317

    Plasmid

    #59571

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCW57-RFP-P2A-MCS

    Plasmid

    #78933

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TALED_Right-mouse-ATP6-1397C-AD

    Plasmid

    #212954

    Purpose

    Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSSRB070_pAC8-hsCRBN

    Plasmid

    #218789

    Purpose

    Insect cell expression vector for FLAG-TEV-Spy-hsCRBN

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    Protein cereblon (CRBN Human)
    

    Tags

    Flag-TEV-Spy

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-ApaLID211A-HA

    Plasmid

    #211459

    Purpose

    Express mitochondrial localized ApaLI with D211A mutation

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-D211A
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D211A

    Promoter

    CMV

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP‐RNASEH2B

    Plasmid

    #108697

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2B (RNASEH2B Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-mNINJ1-GFP

    Plasmid

    #208776

    Purpose

    MSCV vector expressing WT mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234448

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJWV102-wtcas9sp

    Plasmid

    #99612

    Purpose

    Plasmid containing wild-type cas9sp

    Depositor

    Jan-Willem Veening, Morten Kjos

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    cas9sp
    

    Use

    Synthetic Biology

    Promoter

    PczcD

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSAM_Cam

    Plasmid

    #91570

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

32. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFA6a-6xGLY-FLAG-kanMX6

    Plasmid

    #20751

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Expression

    Yeast

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Mutation

    D908A

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GAL4BD-VP16/pDON207

    Plasmid

    #201230

    Purpose

    GAL4-VP16 transcription factor

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    GAL4BD-VP16
    

    Expression

    Plant

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSRK2706

    Plasmid

    #78571

    Purpose

    Expresses untagged human rhinovirus 3C protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    human rhinovirus 3C protease (HRVBgp1 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. His-CL7-2NLS-SpyCas9(dS)-A22p3star

    Plasmid

    #222970

    Purpose

    Bacterial expression of A22p-fused SpyCas9 construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His-CL7-2NLS-SpyCas9(dS)-A22p3
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103_DYNLL1_Dynein-light

    Plasmid

    #110006

    Purpose

    Protein expression and purification of DYNLL1_Dynein-light

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DYNLL1_Dynein-light (DYNLL1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pFA6a-5FLAG-hphMX6

    Plasmid

    #19342

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    hphMX6
    

    Expression

    Yeast

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAS

    Plasmid

    #206994

    Purpose

    Plasmid expressing both SpCas9 and SaCas9

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
    

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDNR-gRNA

    Plasmid

    #206990

    Purpose

    A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Insert

    Donor plasmid-targeting SaCas9-gRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. peGFP C3-SPG20-PAAA

    Plasmid

    #218581

    Purpose

    Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Proline 172 to Alanine and Tyrosine 174 t…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. peGFP C1-SPG20 SC domain

    Plasmid

    #218580

    Purpose

    Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

    Insert

    SPG20 (SPART Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-417

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. dTAG-IRF8

    Plasmid

    #190620

    Purpose

    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12_G36V

    Expression

    Mammalian

    Mutation

    synonymous mutation for sgRNA resistance for sgIR…

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. TFORF3298

    Plasmid

    #144774

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. Flag-HA-USP2

    Plasmid

    #22577

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP2 (USP2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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