177,170 results
-
Plasmid#86797Purposeretroviral expression of CSF-1DepositorAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
stathmin-GFP
Plasmid#86782PurposeMammalian expression of stathmin-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertstathmin (STMN1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRH2521
Plasmid#84380PurposeExpression of sgRNA from a imyc promoter (Pimyc)DepositorInsertsgRNA cloning site
UseCRISPRExpressionBacterialAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR221 UBR5
Plasmid#81062PurposeGateway entry vector encoding full length UBR5DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
UseCloningMutationK503RPromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
ExpressionBacterialAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NPY-pHTomato
Plasmid#83501PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNPY (NPY Human)
TagspHTomatoExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
h-alpha-ENaC
Plasmid#83430Purposefull length human alpha ENaC with Flag tagDepositorAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB512
Plasmid#74854PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.NRAS G12D
Plasmid#83176PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutationDepositorAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherry_gRNA
Plasmid#80457PurposegRNA expression vector with mCherry transfection controlDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertssp Cas9 gRNA
mCherry
UseCRISPRExpressionMammalianPromoterU6 and chicken β-actin promoterAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-CAG-empty-pgk-hph
Plasmid#48753PurposePiggyBac backbone, CAG promoter, Hygromycin selectionDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF6-myc-TBC1D15
Plasmid#79148Purposemammalian expression of TBC1D15DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTBC1D15 (Tbc1d15 Mouse)
TagsHis, Myc, and V5ExpressionMammalianMutationS616N (please see depositor comment below)PromoterEF-1alphaAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP gRNA (BRDN0000561167)
Plasmid#80034Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-FRT-TO-AID-EGFP
Plasmid#80076PurposeMammalian expression vector for C-terminal AID-EGFP taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAID-EGFP
TagsAID and EGFPExpressionMammalianPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB517
Plasmid#74859PurposeGateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-V2-ORF
Plasmid#73636PurposeGateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).DepositorTypeEmpty backboneTagsVenus fragment 2 (V2)ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RabV CVS-N2c(deltaG)-EGFP
Plasmid#73461PurposeExpresses EGFP for monosynaptic circuit mappingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseNeurotropic virusAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGAMA-empty
Plasmid#74755PurposeMammalian lentiviral vectorDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianPromoterLac promoter, SV40, and T7 promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCR3.1-mIL2
Plasmid#74467Purposeexpression of mouse IL2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL2 (Il2 Mouse)
ExpressionMammalianPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-SLC38A9.1
Plasmid#71855PurposeoverexpressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
TagsFLAGExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE30-hPolB
Plasmid#70761Purposebacterial expression of human DNA Polymerase beta (POLB)DepositorAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-Wt-NmeCas9
Plasmid#71474Purposebacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-WT-NmeCas9
Tags6HisExpressionBacterialAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCherry-C1_SEPT9 i1
Plasmid#71622Purposemammalian expression of human SEPT9 with an mCherry fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeptin 9 (SEPTIN9 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseGateway CloningAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO_FOXA1_#1
Plasmid#70095PurposeExpression of shRNA to human FOXA1, puromycin selectionDepositorAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
hOT-GFP
Plasmid#67848Purposemammalian expression of human oxytocin receptor with GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertoxytocin receptor (OXTR Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-FLEx(FRT)-G
Plasmid#67828PurposeFlp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cellsDepositorInsertrabies glycoprotein
UseAAVExpressionMammalianAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPR124-Tango
Plasmid#66312PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR124 (ADGRA2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 AIF 1-90 mCherry FRB
Plasmid#67530PurposeFor expression of mCherry fused FRB to the mitochondrial innermembraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsAIF a.a. 1 - 90
mCherry
FRB
TagsAIF 1 - 90, FRB, and mCherryExpressionMammalianMutationT->LPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKAR4-NES
TagsCerulean, NES, and cpVenus[E172]ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_iSH2-pMagFast2(3x)-iRFP
Plasmid#67298PurposeBlue light dependent photoswitching systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2-pMagFast2(3x)-iRFP (PIK3R1 Human, Synthetic)
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif5c (Kif5c Rat)
TagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial cellsDepositorInsertCre recombinase
Tags6xHis tagExpressionBacterialPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mucolipin1-pEGFP C3
Plasmid#62960Purposefull length human Mucolipin-1 cloned into pEGFP C3DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMucolipin-1 (MCOLN1 Human)
TagsEGFPExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGateway CloningPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRPF1
Plasmid#65382PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRPF1 (BRPF1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRD1
Plasmid#65375PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRD1 (BRD1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Parkin-IRES-zeo
Plasmid#61727PurposeExpression of human Parkin fused to mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertParkin (PRKN Human)
TagsmCherryExpressionMammalianAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMy-CTCF-GFP
Plasmid#50574PurposeRetroviral vector endoding for mouse CTCF fused to GFPDepositorInsertAmpicilin
UseRetroviralAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GH
Plasmid#60514PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagRFP-T-Mito-7
Plasmid#58023PurposeLocalization: Mitochondria, Excitation: 555, Emission: 584DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMito (COX8A Human)
TagsmTagRFPExpressionMammalianPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by