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Showing: 11301 - 11350 of 17163 results

  1. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid
    #233348
    Purpose
    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted (Cyb5a 95-134) and N538E
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233348-plasmid-map-sequence-id-466824

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  9. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid
    #191753
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1523A>T (p.K508M) + silent mutation c.1515A&…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191753-plasmid-map-sequence-id-382274

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  10. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  11. CLEC1B-His-bio

    Plasmid
    #52329
    Purpose
    Expresses full-length C-type lectin domain family 1 member B ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CLEC1B (CLEC1B Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52329-plasmid-map-sequence-id-81045

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  13. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  17. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid
    #191756
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1948A>C (p.K650Q) + silent mutation c.1938C&…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191756-plasmid-map-sequence-id-382285

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  19. CD69-His-bio

    Plasmid
    #52328
    Purpose
    Expresses full-length C-type lectin domain family 2 member C ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD69 (CD69 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52328-plasmid-map-sequence-id-81044

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  23. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid
    #207769
    Purpose
    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207769-plasmid-map-sequence-id-413794

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  25. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid
    #68218
    Purpose
    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Luc / Renilla / P19
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos / 35S / 35S
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68218-plasmid-map-sequence-id-135582

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  27. Alpha MHC-DN.JNK2

    Plasmid
    #51931
    Purpose
    mammalian expression of dominant negative JNK2
    Depositor
    Jeffery Molkentin
    Article
    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.
    Insert
    DN JNK2 (Mapk9 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    dominant negative: the dual phosphorylation moti…
    Promoter
    cardiac-specific α-myosin heavy chain 5.5 kb pro…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Available Since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  32. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  35. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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  41. Venus-Noxa-4E-pEGFP-C1

    Plasmid
    #166745
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Noxa-4E (PMAIP1 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    April 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    166745-plasmid-map-sequence-id-330212

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  46. pMMK ENTR 3 CMV mito mCherry

    Plasmid
    #206256
    Purpose
    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mito mCherry
    Use
    Multimate/gateway entr 3
    Tags
    COX8 mitochondrial tag
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206256-plasmid-map-sequence-id-406392

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Showing: 11301 - 11350 of 17163 results