2,226 results

1. EGFP-Septin 11 isoform II

   Plasmid

   #120317

   Purpose

   Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-CIB1-mRFP1-MP

   Plasmid

   #58367

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

   Plasmid

   #154780

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   CRFR1 (Crhr1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. VV073: 1xCox8 - GCaMP5G in fubi

   Plasmid

   #58509

   Purpose

   Expresses the calcium sensor GCaMP5G in the mitochondria

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - GCaMP5G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KIF5A-GFP-SspB

   Plasmid

   #214401

   Purpose

   Expresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)

   Depositor

   Liting Duan

   Article

   Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

   Insert

   KIF5A-GFP-SspB (Kif5a Rat, Synthetic)
   

   Use

   Tags

   KIF5A-GFP-SspB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBa.Kif1a 1-396.GFP

   Plasmid

   #45058

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

   Insert

   Kif 1a (Kif1a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   motor domain aa 1-396

   Promoter

   Chicken Beta Actin

   Available since

   Oct. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET15b-His-3C-irisin

   Plasmid

   #122612

   Purpose

   Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

   Depositor

   Harold Erickson

   Article

   Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

   Insert

   irisin (ectodomain of FNDC5) (Fndc5 Human, Mouse, Rat, Bovine)
   

   Use

   Tags

   Nt: histag-3C protease site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 polymerase

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Cx43-7

   Plasmid

   #55023

   Purpose

   Localization: Gap Junctions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1-CAMKIIbeta(T287D)

    Plasmid

    #21223

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIbeta (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CAMKIIbeta(T287D) Lab plasmid CF 0001.

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. GFP-N2-PKCgamma

    Plasmid

    #21204

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKC gamma (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKC gamma (rat). (Lab plasmid WC 0013)

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSV2 neuT

    Plasmid

    #10919

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pMC-6-Cre-GR-9

    Plasmid

    #197723

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Cre recombinase with St-LS1 intron fused to glucocorticoid receptor
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mTagRFP-T-Cx26-7

    Plasmid

    #58007

    Purpose

    Localization: Gap Junctions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Cx26 (Gjb2 Rat)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. FSW-HRP-V5-NLGN2

    Plasmid

    #82545

    Purpose

    Overexpression vector for V5-NLGN2 in neurons

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Use

    Lentiviral

    Tags

    HRP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCF CREB M1

    Plasmid

    #22969

    Depositor

    Marc Montminy

    Article

    Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFlc245

    Plasmid

    #101021

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR2Q024y

    Plasmid

    #101022

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cmV

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRXIP Lamp1-Venus

    Plasmid

    #89937

    Purpose

    Expresses Lamp1 tagged with Venus in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    rat Lamp1 (Lamp1 Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAG-Myc-GRM1-IRES-CyRFP1

    Plasmid

    #195355

    Purpose

    mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    GRM1-IRES-CyRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSV2 neuNT

    Plasmid

    #10918

    Depositor

    Bob Weinberg

    Article

    Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

    Insert

    neuNT (Erbb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2006

    Availability

    Academic Institutions and Nonprofits only

23. pMK279 (mClover-Bsr)

    Plasmid

    #72795

    Purpose

    C-terminal tagging with mClover using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mClover-Bsr
    

    Use

    CRISPR

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX-PGK-LAMP1-EYFP

    Plasmid

    #164215

    Purpose

    pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    PGK

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-EF1a-jGCaMP8s-WPRE

    Plasmid

    #176758

    Purpose

    AAV-mediated expression of GCaMP8s under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Pantelis Tsoulfas, Murray Blackmore, Gregory Petsko

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCDHGC5-EGFP

    Plasmid

    #119933

    Purpose

    Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

    Depositor

    Angel de Blas

    Article

    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

    Insert

    PCDHGC5 (Pcdhgc5 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. E. coli expression: Z-lock cofilin dark state mutant

    Plasmid

    #141135

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D3 deletion, I32F in Zdk2

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF HaloTag-SYB2

    Plasmid

    #197282

    Purpose

    Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYB2 and HaloTag (Vamp2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q76V and F77W

    Promoter

    EF1alpha

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-4T1-14-3-3 gamma GST

    Plasmid

    #13280

    Depositor

    Michael Yaffe

    Article

    Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only