We narrowed to 13,682 results for: sequence
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Plasmid#81241PurposeA ZipGFP-based Caspase-3 protease reporter T2A mCherryDepositorInsertZipGFP with caspase cleavage sequence T2A mCherry
ExpressionMammalianAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^EGFP
Plasmid#174109PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.DepositorInsertCodon optimized VPS13D
TagsEGFPExpressionMammalianMutationWith an internal EGFP tag in aminoacid position 1…PromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
TagsC-terminal FLAG tag with a linkerExpressionBacterialAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-CTwinStrep
Plasmid#45941Purposeuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneTagsPelB and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
Plasmid#31484DepositorInsertEF1s HA NLS Sce(opt) T2A IFP1.4
UseLentiviralTagsHA and NLSMutation"Opt" indicates the I-SceI coding seque…Available SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
TagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianPromoterCMVAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T1
Plasmid#41819PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T1
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
HRE-doUnaG
Plasmid#201713PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequenceDepositorInsertUnaG
ExpressionMammalianAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB
Plasmid#228869PurposePE7 connected to MLH1-SB via 2A sequenceDepositorInsertPE7-2A-MLH1-SB
UseCRISPRAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSC-GFP-NES-EGFP
Plasmid#221810PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).DepositorInsertGFP-NES-EGFP
UseLentiviralTagsNES (internal tag)ExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBigT
Plasmid#21270PurposeUsed for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
VPS13D^Halo
Plasmid#174108PurposeThe insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.DepositorInsertCodon optimized VPS13D
TagsHaloExpressionMammalianMutationWith an internal Halo tag in aminoacid position 1…PromoterCMVAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
Plasmid#50022Purposeexpresses Membrane tagged G-CaMP3 in cells expressing CreDepositorInsertG-CaMP3
UseAAVTagsMARCKS sequence (MGCCFSKT)PromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
TagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianPromoterEF-1aAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterU6Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS
Plasmid#158058PurposeDonor plasmid for chromosomal integrations of any sequence at a locus downstream of the glmS gene in Enterobacteriaceae using kanamycin resistance as a selectable markerDepositorInsertsglmS homologous region 1
glmS homologous region 2 + MCS3
UseSynthetic BiologyAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENV8(WT)-GFPuv
Plasmid#70127PurposeCobalamin riboswitch reporter vector for use in E. coliDepositorInsertwild type environmental cobalamin riboswitch sequence (env8)
UseSynthetic BiologyTagsNoneExpressionBacterialPromotertacAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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mito-mGarnet2
Plasmid#104310Purposecodes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mitoDepositorInsertmito-mGarnet2
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorInsertmitochondria targeting sequence
TagsmEosEMExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1147 GFP-TDP43
Plasmid#118797PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP (TARDBP Human)
UseLentiviralAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
EMTB-3XGFP
Plasmid#26741DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags3x EGFP and MycExpressionMammalianMutationOnly contains the microtubule binding-domain of e…Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mCherry
Plasmid#26742DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags2x mCherry and MycExpressionMammalianMutationMicrotubule binding-domain of ensconsin (amino ac…Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only