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164,127 results

Showing: 9201 - 9240 of 164127 results

  1. plix403-V5-N-mCherry

    Plasmid
    #223956
    Purpose
    Gateway cloning of genes of interest with an N-terminal V5-mCherry fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Roko Žaja, Karla Feijs
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-mCherry
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223956-plasmid-map-sequence-id-450836

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  2. APLP2-bio-His

    Plasmid
    #51642
    Purpose
    Expresses full-length Amyloid-like protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APLP2 (APLP2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51642-plasmid-map-sequence-id-78964

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  3. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  4. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217342-plasmid-map-sequence-id-429351

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  8. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  10. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  17. mito-meGFP

    Plasmid
    #172481
    Purpose
    meGFP targeted to the mitochondrial matrix with a human COX8 presequence
    Depositor
    Thomas Schwarz
    Insert
    Mito
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    172481-plasmid-map-sequence-id-345344

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  20. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177435
    Purpose
    To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.
    Depositor
    David Andrews
    Insert
    Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177435-plasmid-map-sequence-id-348466

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  28. pEF274 SVAfw

    Plasmid
    #178192
    Purpose
    The SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.
    Depositor
    Elizabeth Fisher
    Insert
    SVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism
    Use
    Linear bac vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    178192-plasmid-map-sequence-id-353384

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  31. pLenti.PGK.LifeAct-Ruby.W

    Plasmid
    #51009
    Purpose
    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter
    Depositor
    Rusty Lansford
    Insert
    LifeAct-mRFPruby
    Use
    Lentiviral
    Tags
    LifeAct
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51009-plasmid-map-sequence-id-339916

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  34. LPAR1-Tango

    Plasmid
    #66418
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    LPAR1 (LPAR1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66418-plasmid-map-sequence-id-244390

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  35. pBABE puro K-Ras V12

    Plasmid
    #9052
    Purpose
    Retroviral expression of a constitutively active form of K-Ras
    Depositor
    William Hahn
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    G12V. Constitutively active.
    Promoter
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    9052-plasmid-map-sequence-id-219889

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Showing: 9201 - 9240 of 164127 results