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Showing: 11751 - 11800 of 21628 results

  9. pDA2ScIfm

    Plasmid
    #193786
    Purpose
    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTATAA synthetic promoter carrying binding sites…
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    193786-plasmid-map-sequence-id-389837

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  17. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLLacO-1 and PLTetO-1
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193792-plasmid-map-sequence-id-390100

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  22. pAAV/CaMKIIa::iLMO2

    Plasmid
    #129481
    Purpose
    AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter
    Depositor
    Robert Gross
    Article
    Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10
    Insert
    iLMO2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    129481-plasmid-map-sequence-id-385172

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  24. p21StBD

    Plasmid
    #190269
    Purpose
    Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.
    Depositor
    Assaf Mosquna
    Article
    Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2
    Type
    Empty backbone
    Use
    Synthetic Biology; Yeast genome-integrating vector
    Tags
    GAL4-BD
    Expression
    Mutation
    Promoter
    TDH3
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190269-plasmid-map-sequence-id-377250

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  25. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid
    #185968
    Purpose
    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Molecular IMPLY gate
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    185968-plasmid-map-sequence-id-370628

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  27. pNB_EA_R3tdTomatoi2

    Plasmid
    #185969
    Purpose
    Expresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Pr-i2rbs-tdTomato cassette in reverse direction in Network Brick
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PR
    Available since
    Aug. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185969-plasmid-map-sequence-id-370632

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  30. pTS1043

    Plasmid
    #169621
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA) .
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169621-plasmid-map-sequence-id-366769

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  31. pTS1042

    Plasmid
    #169620
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169620-plasmid-map-sequence-id-365622

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  32. pCMV_NFL(K468TAG)-FLAG

    Plasmid
    #182658
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182658-plasmid-map-sequence-id-363090

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  33. pCMV_NFL(K211TAG)-FLAG

    Plasmid
    #182655
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K211TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182655-plasmid-map-sequence-id-363059

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  34. pCMV_NFL(R438TAG)-FLAG

    Plasmid
    #182657
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    R438TAG
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182657-plasmid-map-sequence-id-363055

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  35. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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  36. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  37. pCMV_FLAG-NFL(K468TAG)

    Plasmid
    #182663
    Purpose
    Expresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
    Use
    Tags
    FLAG tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    K468TAG
    Promoter
    CMV
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182663-plasmid-map-sequence-id-362921

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  39. pPOLY_new

    Plasmid
    #179276
    Purpose
    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d
    Inserts
    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    Use
    Synthetic Biology
    Tags
    6xHIS and Strep-Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lacO
    Available since
    April 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179276-plasmid-map-sequence-id-358730

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Showing: 11751 - 11800 of 21628 results