159,589 results

1. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

   Plasmid

   #52092

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_HaloTag7_LamB1

   Plasmid

   #175528

   Purpose

   Expression of HaloTag7 at the nuclear lamina of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag7-LamB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIH-TOMM20-Halo

   Plasmid

   #111626

   Purpose

   Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   TOMM20 (Tomm20 Mouse)
   

   Use

   Retroviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCD1C

   Plasmid

   #102954

   Purpose

   gene cloning of human CD1C

   Depositor

   Lucia Mori, Gennaro De Libero

   Article

   Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

   Insert

   CD1C cDNA (CD1C Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

   Plasmid

   #83030

   Purpose

   Bacterial expression vector for split sfCherry screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry(1-10)_32aalinker_sfCherry(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. E[beta]C

   Plasmid

   #24312

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-cMSP1E3D1

   Plasmid

   #199772

   Purpose

   Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

   Insert

   cMSP1E3D1
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   Extended cMSP1D1; external His-Tags flanking the …

   Promoter

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 3xF-dyskerin

   Plasmid

   #126870

   Purpose

   Expresses 3xFlag dsykerin

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   Dyskerin
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYHY3

    Plasmid

    #216109

    Purpose

    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.

    Depositor

    Shannon Sirk

    Article

    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:

    Insert

    NanoLuc
    

    Use

    Synthetic Biology; E. coli-bacteroides shuttle ve…

    Tags

    3xFLAG tag and BT3630 signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    P1TDP-A21

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-CBE6c-IVT

    Plasmid

    #215835

    Purpose

    Template for in vitro transcription of CBE6c (with SpCas9)

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c-SpCas9-2xUGI
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid

    #177186

    Purpose

    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

    Plasmid

    #40809

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S218E, S222D

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

14. medSIIN

    Plasmid

    #185664

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

    Plasmid

    #29654

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    10xAsn, 6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-HA_WT RAD23A

    Plasmid

    #201458

    Purpose

    Expresses human RAD23A with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJM.677

    Plasmid

    #108530

    Purpose

    AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    AraCAM + AraE + PBAD-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AraC: L133F E165L E169G C280L

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GST-TAX1BP1

    Plasmid

    #171423

    Purpose

    For the purifcation of TAX1BP1 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mPA-GFP-CD81-10

    Plasmid

    #57124

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

23. HRE-dUnaG

    Plasmid

    #201711

    Purpose

    Hypoxia responsive promoter driving destablized UnaG fluorescent protein

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-ERK-SPARK

    Plasmid

    #106921

    Purpose

    Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    ERK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-Rab5A Q79L

    Plasmid

    #28046

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Use

    Tags

    EGFP on N-term

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-FKBP-SAC1deltaTMD

    Plasmid

    #108132

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-521)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCiPS

    Plasmid

    #60011

    Purpose

    Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLVX-TetOne-Puro-MCL1

    Plasmid

    #202663

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. miR155 in pcDNA3.1

    Plasmid

    #78126

    Purpose

    Mammalian expression of miR155

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    miR155 (MIR155 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC19-mAirn-R-loop

    Plasmid

    #134900

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mAirn promoter (Airn Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTOPO-MCL1

    Plasmid

    #21605

    Depositor

    Ulrich Maurer

    Article

    Maurer et al Mol Cell. 2006 Mar 17. 21(6):749-60.

    Insert

    MCL-1 (MCL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert ends with a STOP codon, no V5-His-tag.

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pAAV-Ucp1-Cre-miR122

    Plasmid

    #228408

    Purpose

    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Inserts

    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Ucp1 enhancer-promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB-mTSPAN4-GFP

    Plasmid

    #210329

    Purpose

    Overexpression of mTSPAN4

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mTSPAN4 (Tspan4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ER-moxMaple3

    Plasmid

    #120885

    Purpose

    mammalian expression of ER localized moxMaple3

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

    Insert

    moxMaple3
    

    Use

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Mito-SNAP

    Plasmid

    #206480

    Purpose

    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHLmMBP-8

    Plasmid

    #72347

    Purpose

    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Use

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. ERK-KTR-mStrawberry reporter

    Plasmid

    #158686

    Purpose

    This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

    Plasmid

    #215008

    Purpose

    Express a Lysophagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMXsIP GFP-VPS33A

    Plasmid

    #67022

    Purpose

    human VPS33A ORF was inserted into pMXsIP SPGFP-GS Ci2 vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Vacuolar protein sorting 33 homolog A (S. cerevisiae) (VPS33A) (VPS33A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

46. Human SERCA2a (pcDNA3.1+)

    Plasmid

    #75187

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. PS-CFP2-Tubulin-6

    Plasmid

    #57251

    Purpose

    Localization: Microtubules, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    NM_006082.2 (TUBA1B Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

48. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

    Plasmid

    #85717

    Purpose

    Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Sp dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. p5068 pGEX-6P-1 Brd4 full-length

    Plasmid

    #14447

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 (BRD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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