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Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-GFP-C1 (w392-1)
Plasmid#17396PurposeGateway entry vector for N-term eGFP fusion, frame C1DepositorInsertGFP
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-Co-InCreN
Plasmid#51267PurposeThe N-terminal component of the Co-InCre system, expresses the N-Cre-N-gp41-1 fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreCDepositorInsertCo-InCreN
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Co-InCreC
Plasmid#51268PurposeThe C-terminal component of the Co-InCre system, expresses the C-gp41-1-C-Cre fusion that generates functional Cre by protein trans-splicing upon reacting with Co-InCreNDepositorInsertCo-InCreC
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-APEX2
Plasmid#215555PurposeStable, inducible expression in lysosomeDepositorInsertLAMP1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-ALFA
Plasmid#198301PurposeProduces PVCs retargeted with ALFA tagDepositorInsertPVCpnf_pvc13-ALFA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPthl_fUAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::Halo7
Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-rtTR-KRAB-2SM2
Plasmid#11779PurposeTet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cyto-APEX2
Plasmid#215552PurposeStable, inducible expression in cytoplasmDepositorInsertEGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQCXP CMV/TO DEST (w360-1)
Plasmid#17386PurposeRetroviral Gateway destination vector, Expression, CMV/TO promoter, PuroDepositorTypeEmpty backboneUseRetroviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBS hTSP-5
Plasmid#13002DepositorInsertCOMP (COMP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti X1 Puro empty (w610-1)
Plasmid#20953Purpose3rd gen lentiviral empty control vector, PuroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiALFAtagNb-TM
Plasmid#198307PurposeDisplays anti-ALFA nanobody from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiALFAtagNb-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-hXRCC1
Plasmid#52274PurposeBacterial expression of human XRCC1 with N-terminal GST-tagDepositorInsertXRCC1 (XRCC1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1-HiBiT
Plasmid#198310PurposeLoads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains PnfDepositorInsertPdp1-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIK
Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-Flag-HA
Plasmid#101766PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion proteinDepositorTypeEmpty backboneUseTagsFlag-HAExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLC-284
Plasmid#124294PurposeA low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.DepositorInsertvsfGFP-9
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-StagRFP
Plasmid#173014PurposeRationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.DepositorInsertStagRFP
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tg (mpx:mCherry-2A-Rac2D57N)
Plasmid#58934Purposeexpresses dominant negative rac2 tagged with mcherry in neutrophilsDepositorInsertmcherry-2a-Rac2D57N (rac2 Zebrafish)
UseTagsmcherry2aExpressionMutationchanged Aspartate 57 to AsparaginePromotermpxAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiHistoneNb
Plasmid#198293PurposeProduces PVCs retargeted with A9 anti-histone nanobodyDepositorInsertPVCpnf_pvc13-antiHistoneNb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB-2SM2
Plasmid#11652PurposeTet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-cp6
Plasmid#134938PurposeLow phosphate inducible circular permutation of GFPDepositorInsertGFPcp6
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
YSBE 610 : pHYG_6xHA
Plasmid#92091PurposePlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selectionDepositorInsert6xHA
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yAT1.03
Plasmid#132781PurposeFRET biosensor for ATP measurements in budding yeastDepositorInsertyAT1.03 ymTq2-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tubulin K40A
Plasmid#105304PurposeTo study effects of acetylation-null tubulinDepositorInsertalpha tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40APromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRGD-TcR
Plasmid#74110PurposePlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sitesDepositorInserttetA(C)
UseBacterial vector based on pmb1 repliconTagsExpressionMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-HygTK-L2
Plasmid#141006PurposeMutisite Gateway mediated vector constructionDepositorInsertHygTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4-mCherry
Plasmid#80269Purposemammalian expression plasmid for Plk4-mCherryDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorInsertsI-Sce I Nuclease
BFP
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSIM24 KanR
Plasmid#67583PurposePlant Gene Expression VectorDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterM24Available sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
Endo-APEX2
Plasmid#215553PurposeStable, inducible expression in endosomeDepositorInsertEGFP-2xFYVE-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb
Plasmid#198292PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobodyDepositorInsertPVCpnf_pvc13-antiMouseMHCIINb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hBcan
Plasmid#18966DepositorInsertBrevican (BCAN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF Synaptophysin-GCamp6s
Plasmid#188981PurposePresynaptic fluorescent Ca2+ indicatorDepositorInsertSynaptophysin (Syp Rat, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiEEscFv-TM
Plasmid#198304PurposeDisplays anti-EE scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiEEscFv-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry Tensin3
Plasmid#105301PurposeTo study localization and function of Tensin 3 with EGFP Tensin1DepositorInsertTensin3 (TNS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAXY0006
Plasmid#205575PurposeGlucos(1-231)-NpuDnaE(N)DepositorInsertsGlucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_FLAG-pvc2
Plasmid#198285PurposeContains an external epitope tag on the sheath protein; for immunofluorescence-based binding assaysDepositorInsertPVCpnf_FLAG-pvc2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK485 (3mAID-Hygro)
Plasmid#214397Purpose3mAID for N-terminal taggingDepositorInsert3mAID-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only