159,258 results

1. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

   Plasmid

   #159904

   Purpose

   Mutagenesis of Kcnn3

   Depositor

   Larry Zweifel

   Insert

   Kcnn3 (Kcnn3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDB327

   Plasmid

   #113671

   Purpose

   expresses Ulp1_R3 in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Ulp1_R3
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   contains solubility enhancing mutations

   Promoter

   T7

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLib6.4B-Aalb_743

   Plasmid

   #176676

   Purpose

   Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2+p35SoTMV

   Plasmid

   #170876

   Purpose

   Phytobrick - p35SoTMV

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   p35SoTMV
   

   Use

   Tags

   Expression

   Plant

   Mutation

   214C>T and 229C>T to remove BbsI restrictio…

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-GFP-Syntaxin1A

   Plasmid

   #170871

   Purpose

   Mammalian expression of His-GFP-Syntaxin1A.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Syntaxin 1A (Stx1a Mouse)
   

   Use

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEMB44 / Mouse_LRRK2_1_342

   Plasmid

   #40555

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pETSXM2-pLacI-mtrCAB

   Plasmid

   #174618

   Purpose

   Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

   Depositor

   I-Son Ng

   Article

   I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

   Insert

   mtrCAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacI

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRRK2_1867_2136

   Plasmid

   #40408

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Expression

   Mutation

   WT aa1867_2136

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pMSCVpuro-tdTomato

   Plasmid

   #97079

   Purpose

   Retroviral plasmid containing tdTomato for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Tags

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEMB018 / LRRK2_BC_1847_2350

    Plasmid

    #40533

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Mutation

    WT; aa1847_2350

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA-3-VEGFR3

    Plasmid

    #119230

    Purpose

    Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

    Depositor

    Kari Alitalo

    Insert

    VEGFR3 (FLT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PA-mCherry1-C1

    Plasmid

    #54495

    Purpose

    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-IRES-Neo

    Plasmid

    #28019

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tdEos-Integrin-Beta1-N-18

    Plasmid

    #57627

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    Integrin-Beta1 (ITGB1 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pWT019a

    Plasmid

    #107893

    Purpose

    W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PTetO-sd2U-Cas9, PLacO-sgRNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PYL1_nTEVp

    Plasmid

    #119213

    Purpose

    Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nTEVp in sufion with PYL1
    

    Use

    Tags

    AU1 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

    Plasmid

    #198417

    Purpose

    Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Dronpa-H3.3-N-14

    Plasmid

    #57278

    Purpose

    Localization: Nucleus/Histones, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    H3.3 (H3f3b Mouse)
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. PS-CFP2-CENPA-N-22

    Plasmid

    #57218

    Purpose

    Localization: Nucleus, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    CENPA (CENPA Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. PB CAG-sfGFP

    Plasmid

    #133568

    Purpose

    CAG sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

    Plasmid

    #163047

    Purpose

    GCaMPer calcium sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mGeos2-VEL-PMP-N-10

    Plasmid

    #57557

    Purpose

    Localization: Peroxisomes, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    mGeos2-VEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. m168

    Plasmid

    #34886

    Depositor

    Irvin Chen

    Article

    Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

    Insert

    Sindbis virus envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ABI_cTEVp

    Plasmid

    #119214

    Purpose

    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cTEVp in fusion with ABI
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPD95_25

    Plasmid

    #1486

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, ATG-SecSig, pes10

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pT2K-CAGGS-T2TP

    Plasmid

    #114725

    Purpose

    expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAG-MCP-SRSF1-V5-mCherry

    Plasmid

    #235088

    Purpose

    SRSF1 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

    Plasmid

    #235086

    Purpose

    GFP splicing reporter with CORO1B intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    custom bidirectional

    Available since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGY037 BSMV-gamma-AmCyan

    Plasmid

    #234818

    Purpose

    To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCB094 FoMV-sgLw

    Plasmid

    #234816

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbLw
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJPC596 FoMV-AmCyan

    Plasmid

    #234812

    Purpose

    To express flourescent protein AmCyan from the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEE082 FoMV

    Plasmid

    #234811

    Purpose

    To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF1977

    Plasmid

    #144120

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX1 (TBX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF0432

    Plasmid

    #143877

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARHGAP35 (ARHGAP35 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HDM_RSV_A2_F

    Plasmid

    #237351

    Purpose

    Expression plasmid for RSV A2 F

    Depositor

    Jesse Bloom

    Article

    Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

    Insert

    RSV A2 F human codon optimized from Genbank Accesion QGW56794.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge