Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,333 results

Showing: 9881 - 9920 of 159333 results

  1. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

    Enlarge

  20. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2 (NES Synthetic)
    Use
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

    Enlarge

  24. AiP14870 - pAAV-AiE0452h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4870)

    Plasmid
    #224186
    Purpose
    AiE0452h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs
    Depositor
    Jonathan Ting, The Allen Institute for Brain Science
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224186-plasmid-map-sequence-id-450138

    Enlarge

  30. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170442-plasmid-map-sequence-id-337888

    Enlarge

  33. pENN.AAV.CB7.CI.eGFP.WPRE.rBG

    Plasmid
    #105542
    Purpose
    AAV expression of EGFP from CB7 promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB7
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105542-plasmid-map-sequence-id-201063

    Enlarge

  34. pAAV-Ucp1-Cre-miR122

    Plasmid
    #228408
    Purpose
    Expression of Cre recombinase from a rat Ucp1 enhancer-promoter. Contains miR122 target sequences that suppress expression in hepatocytes.
    Depositor
    Joerg Heeren
    Article
    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1
    Inserts
    cre recombinase with SV40 NLS
    Cre recombinase with SV40 NLS
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    rat Ucp1 enhancer-promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    228408-plasmid-map-sequence-id-459311

    Enlarge

  35. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

Showing: 9881 - 9920 of 159333 results